5OFO

Cryo EM structure of the E. coli disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state, bound to the model substrate casein

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0009408	biological_process	response to heat
A	0016887	molecular_function	ATP hydrolysis activity
A	0042026	biological_process	protein refolding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0009408	biological_process	response to heat
B	0016887	molecular_function	ATP hydrolysis activity
B	0042026	biological_process	protein refolding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0009408	biological_process	response to heat
C	0016887	molecular_function	ATP hydrolysis activity
C	0042026	biological_process	protein refolding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0009408	biological_process	response to heat
D	0016887	molecular_function	ATP hydrolysis activity
D	0042026	biological_process	protein refolding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0009408	biological_process	response to heat
E	0016887	molecular_function	ATP hydrolysis activity
E	0042026	biological_process	protein refolding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0009408	biological_process	response to heat
F	0016887	molecular_function	ATP hydrolysis activity
F	0042026	biological_process	protein refolding

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	binding site for residue AGS C 901

Chain	Residue
B	ARG332
C	ALA214
C	ILE349
C	PRO387
C	VAL180
C	GLU207
C	PRO208
C	GLY209
C	VAL210
C	GLY211
C	LYS212
C	THR213

---

site_id	AC2
Number of Residues	12
Details	binding site for residue AGS C 902

Chain	Residue
B	GLU752
B	ARG756
C	ARG569
C	ILE571
C	GLY608
C	VAL609
C	GLY610
C	LYS611
C	THR612
C	GLU613
C	ALA814
C	ARG815

---

site_id	AC3
Number of Residues	17
Details	binding site for residue AGS F 901

Chain	Residue
E	ALA327
E	GLU330
E	ARG331
F	PRO179
F	VAL180
F	ILE181
F	ARG183
F	GLU207
F	PRO208
F	GLY209
F	VAL210
F	GLY211
F	LYS212
F	THR213
F	ALA214
F	ILE349
F	PRO387

---

site_id	AC4
Number of Residues	11
Details	binding site for residue AGS F 902

Chain	Residue
F	THR607
F	GLY608
F	VAL609
F	GLY610
F	LYS611
F	THR612
F	GLU613
F	ARG631
F	LEU766
F	ILE774
F	ARG815

---

site_id	AC5
Number of Residues	11
Details	binding site for residue AGS E 901

Chain	Residue
D	ARG331
E	ILE181
E	GLY209
E	VAL210
E	GLY211
E	LYS212
E	THR213
E	ALA214
E	ASP278
E	ILE349
E	LEU353

---

site_id	AC6
Number of Residues	11
Details	binding site for residue AGS E 902

Chain	Residue
D	GLU752
E	ARG569
E	THR607
E	GLY608
E	VAL609
E	GLY610
E	LYS611
E	THR612
E	GLU613
E	ASN719
E	ARG815

---

site_id	AC7
Number of Residues	14
Details	binding site for residue AGS D 901

Chain	Residue
C	ARG331
C	ARG332
D	PRO179
D	ARG183
D	GLU207
D	GLY209
D	VAL210
D	GLY211
D	LYS212
D	THR213
D	ALA214
D	ILE349
D	PRO387
D	ASP388

---

site_id	AC8
Number of Residues	12
Details	binding site for residue AGS D 902

Chain	Residue
D	ILE774
C	ASP695
C	ARG750
C	GLU752
D	ILE571
D	THR607
D	GLY608
D	VAL609
D	GLY610
D	LYS611
D	THR612
D	GLU613

---

site_id	AC9
Number of Residues	12
Details	binding site for residue AGS B 901

Chain	Residue
A	ARG331
A	ARG332
B	VAL180
B	GLU207
B	PRO208
B	VAL210
B	GLY211
B	LYS212
B	THR213
B	ALA214
B	ILE349
B	PRO387

---

site_id	AD1
Number of Residues	11
Details	binding site for residue AGS B 902

Chain	Residue
B	ARG569
B	GLY608
B	VAL609
B	GLY610
B	LYS611
B	THR612
B	GLU613
B	ASN719
B	ILE774
B	ALA814
B	ARG815

---

site_id	AD2
Number of Residues	14
Details	binding site for residue AGS A 901

Chain	Residue
A	ASP178
A	VAL180
A	ILE181
A	ARG183
A	GLY209
A	VAL210
A	GLY211
A	LYS212
A	THR213
A	ALA214
A	ILE349
A	LEU353
A	PRO387
F	ARG331

---

Functional Information from PROSITE/UniProt

site_id	PS00870
Number of Residues	13
Details	CLPAB_1 Chaperonins clpA/B signature 1. DAGNMLKPaLarG

Chain	Residue	Details
C	ASP294-GLY306

---

site_id	PS00871
Number of Residues	19
Details	CLPAB_2 Chaperonins clpA/B signature 2. RIDmSEFmEKhSvSRLvGA

Chain	Residue	Details
C	ARG631-ALA649

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	84
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1224
Details	Region: {"description":"Linker"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1260
Details	Region: {"description":"NBD2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	546
Details	Region: {"description":"C-terminal"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	804
Details	Coiled coil: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---