4NCN

Crystal structure of eukaryotic translation initiation factor eIF5B (517-858) from Chaetomium thermophilum in complex with GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Eukaryotic translation initiation factor 5B-like protein	polymer	457	50972.5	2	UniProt (G0S8G9) Pfam (PF00009) Pfam (PF11987) In PDB	Chaetomium thermophilum var. thermophilum
2	A, B	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A, B	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6	B	ACETIC ACID	non-polymer		60.1	1	Chemie (ACY)
7		water	water		18.0	508	Chemie (HOH)

Sequence modifications

A, B: 517 - 970 (UniProt: G0S8G9)

PDB	External Database	Details
Ser 514	-	expression tag
His 515	-	expression tag
Met 516	-	expression tag
Val 576	-	SEE REMARK 999
Val 577	-	SEE REMARK 999
Asn 578	-	SEE REMARK 999
Lys 579	-	SEE REMARK 999
Asp 580	-	SEE REMARK 999
Gly 581	-	SEE REMARK 999
Lys 582	-	SEE REMARK 999
Phe 583	-	SEE REMARK 999
Glu 584	-	SEE REMARK 999
Phe 585	-	SEE REMARK 999
Lys 586	-	SEE REMARK 999
Val 587	-	SEE REMARK 999
Pro 588	-	SEE REMARK 999
Gly 589	-	SEE REMARK 999
Leu 590	-	SEE REMARK 999
Leu 591	-	SEE REMARK 999
Ile 592	-	SEE REMARK 999
Ile 593	-	SEE REMARK 999
Asp 594	-	SEE REMARK 999
Thr 595	-	SEE REMARK 999
Pro 596	-	SEE REMARK 999
Gly 597	-	SEE REMARK 999
His 598	-	SEE REMARK 999
Glu 599	-	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	101945.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1845.7
All*	Total formula weight	103790.7

*Water molecules are not included.