4NCN
Crystal structure of eukaryotic translation initiation factor eIF5B (517-858) from Chaetomium thermophilum in complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918410 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.410, 114.840, 65.880 |
Unit cell angles | 90.00, 102.41, 90.00 |
Refinement procedure
Resolution | 46.645 - 1.870 |
R-factor | 0.1656 |
Rwork | 0.164 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n3n |
RMSD bond length | 0.019 |
RMSD bond angle | 1.773 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.645 | 1.970 | |
High resolution limit [Å] | 1.870 | 50.000 | 1.870 |
Rmerge | 0.044 | 0.620 | |
Number of reflections | 66297 | ||
<I/σ(I)> | 20.53 | 2.38 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Hepes, 13 % PEG 4000, 100 mM NaOAc, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |