4NCN
Crystal structure of eukaryotic translation initiation factor eIF5B (517-858) from Chaetomium thermophilum in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.410, 114.840, 65.880 |
| Unit cell angles | 90.00, 102.41, 90.00 |
Refinement procedure
| Resolution | 46.645 - 1.870 |
| R-factor | 0.1656 |
| Rwork | 0.164 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n3n |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.773 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.645 | 1.970 | |
| High resolution limit [Å] | 1.870 | 50.000 | 1.870 |
| Rmerge | 0.044 | 0.620 | |
| Number of reflections | 66297 | ||
| <I/σ(I)> | 20.53 | 2.38 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Hepes, 13 % PEG 4000, 100 mM NaOAc, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
