8D3Q
| Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/NoPAM prespacer | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endonuclease Cas2, CRISPR-associated exonuclease Cas4, ... | Authors: | Dhingra, Y, Suresh, S.K, Juneja, P, Sashital, D.G. | Deposit date: | 2022-06-01 | Release date: | 2022-11-02 | Last modified: | 2024-06-12 | Method: | ELECTRON MICROSCOPY (3.9 Å) | Cite: | PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation. Mol.Cell, 82, 2022
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8D3M
| Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/Processed prespacer | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endonuclease Cas2, CRISPR-associated exonuclease Cas4, ... | Authors: | Dhingra, Y, Suresh, S.K, Juneja, P, Sashital, D.G. | Deposit date: | 2022-06-01 | Release date: | 2022-11-02 | Last modified: | 2022-11-30 | Method: | ELECTRON MICROSCOPY (3.41 Å) | Cite: | PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation. Mol.Cell, 82, 2022
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8D3L
| Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/PAM prespacer | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endonuclease Cas2, CRISPR-associated exonuclease Cas4, ... | Authors: | Dhingra, Y, Suresh, S.K, Juneja, P, Sashital, D.G. | Deposit date: | 2022-06-01 | Release date: | 2022-11-02 | Last modified: | 2024-06-12 | Method: | ELECTRON MICROSCOPY (3.49 Å) | Cite: | PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation. Mol.Cell, 82, 2022
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8D3P
| Type I-C Cas4-Cas1-Cas2 complex bound to half-site integration intermediate (HSI) | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endonuclease Cas2, CRISPR-associated exonuclease Cas4, ... | Authors: | Dhingra, Y, Suresh, S.K, Juneja, P, Sashital, D.G. | Deposit date: | 2022-06-01 | Release date: | 2022-11-02 | Last modified: | 2022-11-30 | Method: | ELECTRON MICROSCOPY (4.26 Å) | Cite: | PAM binding ensures orientational integration during Cas4-Cas1-Cas2-mediated CRISPR adaptation. Mol.Cell, 82, 2022
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5VVL
| Cas1-Cas2 bound to full-site mimic with Ni | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (11-MER), ... | Authors: | Wright, A.V, Knott, G.J, Doxzen, K.D, Doudna, J.A. | Deposit date: | 2017-05-19 | Release date: | 2017-08-02 | Last modified: | 2023-10-04 | Method: | X-RAY DIFFRACTION (3.31 Å) | Cite: | Structures of the CRISPR genome integration complex. Science, 357, 2017
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5VVK
| Cas1-Cas2 bound to full-site mimic | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (5'-D(*GP*AP*CP*CP*AP*CP*CP*AP*GP*TP*G)-3'), ... | Authors: | Wright, A.V, Knott, G.J, Doxzen, K.D, Doudna, J.A. | Deposit date: | 2017-05-19 | Release date: | 2017-08-02 | Last modified: | 2023-10-04 | Method: | X-RAY DIFFRACTION (2.9 Å) | Cite: | Structures of the CRISPR genome integration complex. Science, 357, 2017
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5VVJ
| Cas1-Cas2 bound to half-site intermediate | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (112-MER), ... | Authors: | Wright, A.V, Knott, G.J, Doxzen, K.W, Doudna, J.A. | Deposit date: | 2017-05-19 | Release date: | 2017-08-02 | Last modified: | 2023-10-04 | Method: | X-RAY DIFFRACTION (3.89 Å) | Cite: | Structures of the CRISPR genome integration complex. Science, 357, 2017
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8K22
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8K23
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8K24
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7MI4
| Symmetrical PAM-PAM prespacer bound Cas4/Cas1/Cas2 complex | Descriptor: | CRISPR-associated endoribonuclease Cas2, CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, DNA (35-MER), ... | Authors: | Hu, C.Y, Ke, A.K. | Deposit date: | 2021-04-16 | Release date: | 2021-11-17 | Method: | ELECTRON MICROSCOPY (3.2 Å) | Cite: | Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas. Nature, 598, 2021
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7MI5
| Asymmetrical PAM-Non PAM prespacer bound Cas4/Cas1/Cas2 complex | Descriptor: | CRISPR-associated endoribonuclease Cas2, CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, DNA (26-MER), ... | Authors: | Hu, C.Y, Ke, A.K. | Deposit date: | 2021-04-16 | Release date: | 2021-11-17 | Method: | ELECTRON MICROSCOPY (3.57 Å) | Cite: | Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas. Nature, 598, 2021
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7MIB
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7MID
| Sub-complex of Cas4-Cas1-Cas2 bound PAM containing DNA | Descriptor: | CRISPR-associated endoribonuclease Cas2, CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, DNA (33-MER), ... | Authors: | Hu, C.Y, Ke, A.K. | Deposit date: | 2021-04-16 | Release date: | 2021-11-17 | Method: | ELECTRON MICROSCOPY (3.56 Å) | Cite: | Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas. Nature, 598, 2021
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7MI9
| Full integration complex of Cas1/Cas2 from Cas4-containing system | Descriptor: | CRISPR-associated endoribonuclease Cas2, CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, DNA (5'-D(P*CP*GP*GP*AP*AP*AP*AP*GP*AP*GP*CP*C)-3'), ... | Authors: | Hu, C.Y, Ke, A.K. | Deposit date: | 2021-04-16 | Release date: | 2021-11-17 | Last modified: | 2024-05-29 | Method: | ELECTRON MICROSCOPY (3.89 Å) | Cite: | Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas. Nature, 598, 2021
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5DS5
| Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... | Authors: | Nunez, J.K, Harrington, L.B, Kranzusch, P.J, Engelman, A.N, Doudna, J.A. | Deposit date: | 2015-09-16 | Release date: | 2015-10-28 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.951 Å) | Cite: | Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature, 527, 2015
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5DQU
| Crystal Structure of Cas-DNA-10 complex | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (5'-D(*GP*AP*GP*TP*CP*GP*AP*TP*GP*CP*TP*TP*TP*TP*T)-3'), ... | Authors: | Wang, J, Li, J, Zhao, H, Sheng, G, Wang, M, Yin, M, Wang, Y. | Deposit date: | 2015-09-15 | Release date: | 2015-11-11 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (4.5 Å) | Cite: | Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell, 163, 2015
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5DS4
| Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER) | Authors: | Nunez, J.K, Harrington, L.B, Kranzusch, P.J, Engelman, A.N, Doudna, J.A. | Deposit date: | 2015-09-16 | Release date: | 2015-10-28 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (3.2 Å) | Cite: | Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature, 527, 2015
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5DQT
| Crystal Structure of Cas-DNA-22 complex | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (33-MER), ... | Authors: | Wang, J, Li, J, Zhao, H, Sheng, G, Wang, M, Yin, M, Wang, Y. | Deposit date: | 2015-09-15 | Release date: | 2015-11-11 | Last modified: | 2024-03-20 | Method: | X-RAY DIFFRACTION (3.1 Å) | Cite: | Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell, 163, 2015
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5DLJ
| Crystal Structure of Cas-DNA-N1 complex | Descriptor: | 39-mer DNA N1-F, 39-mer DNA N1-R, CRISPR-associated endonuclease Cas1, ... | Authors: | Wang, J, Li, J, Zhao, H, Sheng, G, Wang, M, Yin, M, Wang, Y. | Deposit date: | 2015-09-05 | Release date: | 2015-11-11 | Last modified: | 2024-03-20 | Method: | X-RAY DIFFRACTION (2.6 Å) | Cite: | Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell, 163, 2015
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5DQZ
| Crystal Structure of Cas-DNA-PAM complex | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (36-MER), ... | Authors: | Wang, J, Li, J, Zhao, H, Sheng, G, Wang, M, Yin, M, Wang, Y. | Deposit date: | 2015-09-15 | Release date: | 2015-11-11 | Last modified: | 2023-11-08 | Method: | X-RAY DIFFRACTION (2.7 Å) | Cite: | Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. Cell, 163, 2015
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5DS6
| Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with splayed ends | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... | Authors: | Nunez, J.K, Harrington, L.B, Kranzusch, P.J, Engelman, A.N, Doudna, J.A. | Deposit date: | 2015-09-16 | Release date: | 2015-10-28 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (3.352 Å) | Cite: | Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature, 527, 2015
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8FLJ
| Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA | Descriptor: | CRISPR leader and repeat, anti-sense strand of DNA, CRISPR leader, ... | Authors: | Santiago-Frangos, A, Henriques, W.S, Wiegand, T, Gauvin, C, Buyukyoruk, M, Neselu, K, Eng, E.T, Lander, G.C, Wiedenheft, B. | Deposit date: | 2022-12-21 | Release date: | 2023-09-06 | Last modified: | 2023-11-22 | Method: | ELECTRON MICROSCOPY (3.48 Å) | Cite: | Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays. Nat.Struct.Mol.Biol., 30, 2023
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5WFE
| Cas1-Cas2-IHF-DNA holo-complex | Descriptor: | CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... | Authors: | Wright, A.V, Liu, J.J, Nogales, E, Doudna, J.A. | Deposit date: | 2017-07-11 | Release date: | 2017-08-02 | Last modified: | 2024-03-13 | Method: | ELECTRON MICROSCOPY (3.64 Å) | Cite: | Structures of the CRISPR genome integration complex. Science, 357, 2017
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7KFU
| Cas6-RT-Cas1--Cas2 complex | Descriptor: | Cas2, Cas6-RT-Cas1 | Authors: | Hoel, C.M, Wang, J.Y, Doudna, J.A, Brohawn, S.G. | Deposit date: | 2020-10-14 | Release date: | 2021-03-31 | Last modified: | 2024-03-06 | Method: | ELECTRON MICROSCOPY (3.9 Å) | Cite: | Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex. Nat Commun, 12, 2021
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