8FLJ

Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA

Summary for 8FLJ

• Entry DOI: 10.2210/pdb8flj/pdb
• EMDB information: 29280
• Descriptor: CRISPR-associated endonuclease Cas1, Integration host factor subunit alpha, Integration host factor subunit beta, ... (8 entities in total)
• Functional Keywords: dna integration, maintenance of crispr repeat elements, ihf-dna complex, enzymes altering nucleic acid conformation, site specific endodeoxyribonucleases: cleavage is not sequence specific, nucleotidyltransferases, go:0008301, recombination, dna binding protein, hydrolase-dna complex, hydrolase/dna
• Biological source: Pseudomonas aeruginosa PA14; More
• Total number of polymer chains: 14
• Total formula weight: 562661.58

Authors

Santiago-Frangos, A.,Henriques, W.S.,Wiegand, T.,Gauvin, C.,Buyukyoruk, M.,Neselu, K.,Eng, E.T.,Lander, G.C.,Wiedenheft, B. (deposition date: 2022-12-21, release date: 2023-09-06, Last modification date: 2023-11-22)

• Primary citation: Santiago-Frangos, A.,Henriques, W.S.,Wiegand, T.,Gauvin, C.C.,Buyukyoruk, M.,Graham, A.B.,Wilkinson, R.A.,Triem, L.,Neselu, K.,Eng, E.T.,Lander, G.C.,Wiedenheft, B.; Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays.; Nat.Struct.Mol.Biol., 30:1675-1685, 2023; PubMed: 37710013; DOI: 10.1038/s41594-023-01097-2

Experimental method

ELECTRON MICROSCOPY (3.48 Å)