8FLJ
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | CRISPR-associated endonuclease Cas1 | polymer | 341 | 38267.1 | 4 | UniProt (Q02ML7) Pfam (PF01867) | Pseudomonas aeruginosa PA14 | |
| 2 | E, G (E, G) | Integration host factor subunit alpha | polymer | 102 | 11646.3 | 2 | UniProt (Q02NN5) Pfam (PF00216) | Pseudomonas aeruginosa PA14 | IHF-alpha |
| 3 | F, H (F, H) | Integration host factor subunit beta | polymer | 96 | 10805.4 | 2 | UniProt (Q02PW7) Pfam (PF00216) | Pseudomonas aeruginosa PA14 | IHF-beta |
| 4 | I (I) | CRISPR leader, sense strand of DNA | polymer | 139 | 42935.6 | 1 | Pseudomonas aeruginosa PA14 | ||
| 5 | J (J) | CRISPR leader and repeat, anti-sense strand of DNA | polymer | 171 | 52701.6 | 1 | Pseudomonas aeruginosa PA14 | ||
| 6 | K (K) | CRISPR repeat and prespacer, sense strand of DNA | polymer | 59 | 18241.7 | 1 | synthetic construct | ||
| 7 | L (L) | Prespacer, anti-sense strand of DNA | polymer | 27 | 8263.4 | 1 | synthetic construct | ||
| 8 | M, N (M, N) | CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST | polymer | 1076 | 121273.7 | 2 | UniProt (Q02ML8) Pfam (PF21384) Pfam (PF18019) Pfam (PF22590) Pfam (PF21802) | Pseudomonas aeruginosa PA14 |
Sequence modifications
A, B, C, D: 1 - 324 (UniProt: Q02ML7)
E, G: 3 - 102 (UniProt: Q02NN5)
F, H: 3 - 96 (UniProt: Q02PW7)
| PDB | External Database | Details |
|---|---|---|
| Met -16 | - | expression tag |
| Trp -15 | - | expression tag |
| Ser -14 | - | expression tag |
| His -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Gln -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Lys -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Asn -6 | - | expression tag |
| Leu -5 | - | expression tag |
| Tyr -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 562661.6 | |
| All* | Total formula weight | 562661.6 |
