5DS4

Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA

Summary for 5DS4

• Entry DOI: 10.2210/pdb5ds4/pdb
• Related: 4P6I
• Descriptor: CRISPR-associated endonuclease Cas1, CRISPR-associated endoribonuclease Cas2, DNA (28-MER), ... (4 entities in total)
• Functional Keywords: crispr-cas systems, clustered regularly interspaced short palindromic repeats, integrases, endodeoxyribonucleases, endonucleases, dna binding protein, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 8
• Total formula weight: 173874.04

Authors

Nunez, J.K.,Harrington, L.B.,Kranzusch, P.J.,Engelman, A.N.,Doudna, J.A. (deposition date: 2015-09-16, release date: 2015-10-28, Last modification date: 2023-09-27)

• Primary citation: Nunez, J.K.,Harrington, L.B.,Kranzusch, P.J.,Engelman, A.N.,Doudna, J.A.; Foreign DNA capture during CRISPR-Cas adaptive immunity.; Nature, 527:535-538, 2015; PubMed: 26503043; DOI: 10.1038/nature15760

Experimental method

X-RAY DIFFRACTION (3.2 Å)