5DS4
Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-07-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.020, 123.006, 196.012 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.003 - 3.200 |
R-factor | 0.2438 |
Rwork | 0.242 |
R-free | 0.27040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p6i |
RMSD bond length | 0.003 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.003 | 3.360 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.128 | 0.614 |
Number of reflections | 35808 | |
<I/σ(I)> | 6.4 | 1.5 |
Completeness [%] | 99.8 | 99 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM sodium citrate tribasic pH 5.6, 200 mM sodium acetate and 8% PEG 8000 (w/v) |