7MIB

Half integration complex of Cas4/Cas1/Cas2 with Cas4 still on the Non-PAM side

Summary for 7MIB

• Entry DOI: 10.2210/pdb7mib/pdb
• EMDB information: 23839 23840 23843 23845
• Descriptor: DNA (31-MER), CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, CRISPR-associated endoribonuclease Cas2, ... (6 entities in total)
• Functional Keywords: crispr/cas, cas4, pam recognition, half integration, hydrolase-dna complex, hydrolase/dna
• Biological source: Geobacter sulfurreducens; More
• Total number of polymer chains: 10
• Total formula weight: 319505.27

Authors

Hu, C.Y.,Ke, A.K. (deposition date: 2021-04-16, release date: 2021-11-17)

• Primary citation: Hu, C.,Almendros, C.,Nam, K.H.,Costa, A.R.,Vink, J.N.A.,Haagsma, A.C.,Bagde, S.R.,Brouns, S.J.J.,Ke, A.; Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.; Nature, 598:515-520, 2021; PubMed: 34588691; DOI: 10.1038/s41586-021-03951-z

Experimental method

ELECTRON MICROSCOPY (5.8 Å)