7MI4

Symmetrical PAM-PAM prespacer bound Cas4/Cas1/Cas2 complex

Summary for 7MI4

• Entry DOI: 10.2210/pdb7mi4/pdb
• EMDB information: 23839
• Descriptor: CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion, CRISPR-associated endoribonuclease Cas2, DNA (35-MER), ... (5 entities in total)
• Functional Keywords: crispr/cas, cas4, pam recognition, hydrolase-dna complex, hydrolase/dna
• Biological source: Geobacter sulfurreducens; More
• Total number of polymer chains: 8
• Total formula weight: 295456.95

Authors

Hu, C.Y.,Ke, A.K. (deposition date: 2021-04-16, release date: 2021-11-17)

• Primary citation: Hu, C.,Almendros, C.,Nam, K.H.,Costa, A.R.,Vink, J.N.A.,Haagsma, A.C.,Bagde, S.R.,Brouns, S.J.J.,Ke, A.; Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.; Nature, 598:515-520, 2021; PubMed: 34588691; DOI: 10.1038/s41586-021-03951-z

Experimental method

ELECTRON MICROSCOPY (3.2 Å)