Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7RAN

5-HT2AR bound to a novel agonist in complex with a mini-Gq protein and an active-state stabilizing single-chain variable fragment (scFv16) obtained by cryo-electron microscopy (cryoEM)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004930	molecular_function	G protein-coupled receptor activity
A	0004993	molecular_function	G protein-coupled serotonin receptor activity
A	0005886	cellular_component	plasma membrane
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0016020	cellular_component	membrane
B	0003924	molecular_function	GTPase activity
B	0005525	molecular_function	GTP binding
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0019001	molecular_function	guanyl nucleotide binding
B	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0001750	cellular_component	photoreceptor outer segment
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
C	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
C	0007265	biological_process	Ras protein signal transduction
C	0008283	biological_process	cell population proliferation
C	0016020	cellular_component	membrane
C	0030159	molecular_function	signaling receptor complex adaptor activity
C	0044877	molecular_function	protein-containing complex binding
C	0045202	cellular_component	synapse
C	0050909	biological_process	sensory perception of taste
C	0051020	molecular_function	GTPase binding
C	0060041	biological_process	retina development in camera-type eye
C	0070062	cellular_component	extracellular exosome
C	0071380	biological_process	cellular response to prostaglandin E stimulus
C	0071870	biological_process	cellular response to catecholamine stimulus
C	0097381	cellular_component	photoreceptor disc membrane
C	1903561	cellular_component	extracellular vesicle
D	0005515	molecular_function	protein binding
D	0005834	cellular_component	heterotrimeric G-protein complex
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
D	0016020	cellular_component	membrane
D	0031681	molecular_function	G-protein beta-subunit binding
D	0048144	biological_process	fibroblast proliferation
D	0070062	cellular_component	extracellular exosome
D	0071380	biological_process	cellular response to prostaglandin E stimulus
D	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00237
Number of Residues	17
Details	G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASImHLCAISLDRYVaI

Chain	Residue	Details
A	ALA161-ILE177

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
C	LEU70-SER84
C	ILE157-ILE171
C	LEU285-ALA299

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: N-acetylalanine => ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22814378

Chain	Residue	Details
D	ALA2

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Cysteine methyl ester => ECO:0000250\|UniProtKB:P63212

Chain	Residue	Details
D	CYS68
A	ASP172-LYS191
A	PHE255-VAL324

site_id	SWS_FT_FI3
Number of Residues	1
Details	LIPID: S-geranylgeranyl cysteine => ECO:0000250\|UniProtKB:P63212

Chain	Residue	Details
D	CYS68

site_id	SWS_FT_FI4
Number of Residues	47
Details	TOPO_DOM: Extracellular => ECO:0000250

Chain	Residue	Details
A	LEU133-CYS148
A	GLN216-ASN233
A	VAL347-LEU362

site_id	SWS_FT_FI5
Number of Residues	22
Details	TRANSMEM: Helical; Name=3 => ECO:0000250

Chain	Residue	Details
A	ALA149-LEU171

site_id	SWS_FT_FI6
Number of Residues	23
Details	TRANSMEM: Helical; Name=4 => ECO:0000250

Chain	Residue	Details
A	ALA192-LEU215

site_id	SWS_FT_FI7
Number of Residues	20
Details	TRANSMEM: Helical; Name=5 => ECO:0000250

Chain	Residue	Details
A	PHE234-TYR254

site_id	SWS_FT_FI8
Number of Residues	21
Details	TRANSMEM: Helical; Name=6 => ECO:0000250

Chain	Residue	Details
A	LEU325-ALA346

site_id	SWS_FT_FI9
Number of Residues	21
Details	TRANSMEM: Helical; Name=7 => ECO:0000250

Chain	Residue	Details
A	ASN363-ASN384

site_id	SWS_FT_FI10
Number of Residues	3
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P41595

Chain	Residue	Details
A	ASP155
A	THR160
A	LEU229

site_id	SWS_FT_FI11
Number of Residues	1
Details	SITE: Hydrophobic barrier that decreases the speed of ligand binding and dissociation => ECO:0000269\|PubMed:28129538

Chain	Residue	Details
A	LEU229

site_id	SWS_FT_FI12
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:24637012

Chain	Residue	Details
A	SER280

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)