7RAN

5-HT2AR bound to a novel agonist in complex with a mini-Gq protein and an active-state stabilizing single-chain variable fragment (scFv16) obtained by cryo-electron microscopy (cryoEM)

Summary for 7RAN

• Entry DOI: 10.2210/pdb7ran/pdb
• EMDB information: 24378
• Descriptor: 5-hydroxytryptamine receptor 2A, G protein subunit q (Gi2-mini-Gq chimera), Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, ... (6 entities in total)
• Functional Keywords: 5-ht2a receptor, serotonin receptor, g protein, gpcr, novel agonist, cryoem, membrane protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 5
• Total formula weight: 142403.71

Authors

Barros-Alvarez, X.,Kim, K.,Panova, O.,Roth, B.L.,Skiniotis, G. (deposition date: 2021-07-02, release date: 2022-07-06, Last modification date: 2022-11-02)

• Primary citation: Kaplan, A.L.,Confair, D.N.,Kim, K.,Barros-Alvarez, X.,Rodriguiz, R.M.,Yang, Y.,Kweon, O.S.,Che, T.,McCorvy, J.D.,Kamber, D.N.,Phelan, J.P.,Martins, L.C.,Pogorelov, V.M.,DiBerto, J.F.,Slocum, S.T.,Huang, X.P.,Kumar, J.M.,Robertson, M.J.,Panova, O.,Seven, A.B.,Wetsel, A.Q.,Wetsel, W.C.,Irwin, J.J.,Skiniotis, G.,Shoichet, B.K.,Roth, B.L.,Ellman, J.A.; Bespoke library docking for 5-HT 2A receptor agonists with antidepressant activity.; Nature, 610:582-591, 2022; PubMed: 36171289; DOI: 10.1038/s41586-022-05258-z

Experimental method

ELECTRON MICROSCOPY (3.45 Å)