7CFT

Cryo-EM strucutre of human acid-sensing ion channel 1a in complex with snake toxin Mambalgin1 at pH 8.0

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005272	molecular_function	sodium channel activity
A	0006814	biological_process	sodium ion transport
A	0015280	molecular_function	ligand-gated sodium channel activity
A	0016020	cellular_component	membrane
B	0005272	molecular_function	sodium channel activity
B	0006814	biological_process	sodium ion transport
B	0015280	molecular_function	ligand-gated sodium channel activity
B	0016020	cellular_component	membrane
C	0005272	molecular_function	sodium channel activity
C	0006814	biological_process	sodium ion transport
C	0015280	molecular_function	ligand-gated sodium channel activity
C	0016020	cellular_component	membrane
D	0005576	cellular_component	extracellular region
D	0035821	biological_process	modulation of process of another organism
D	0090729	molecular_function	toxin activity
D	0099106	molecular_function	ion channel regulator activity
E	0005576	cellular_component	extracellular region
E	0035821	biological_process	modulation of process of another organism
E	0090729	molecular_function	toxin activity
E	0099106	molecular_function	ion channel regulator activity
F	0005576	cellular_component	extracellular region
F	0035821	biological_process	modulation of process of another organism
F	0090729	molecular_function	toxin activity
F	0099106	molecular_function	ion channel regulator activity

Functional Information from PROSITE/UniProt

site_id	PS01206
Number of Residues	21
Details	ASC Amiloride-sensitive sodium channels signature. YsiTaCridCeTryLVenCnC

Chain	Residue	Details
A	TYR305-CYS325

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	SITE: Important residue for inhibition of rat ASIC1a => ECO:0000269|PubMed:26680001

Chain	Residue	Details
D	PHE27
F	ARG28
F	ILE33
F	LEU34
D	ARG28
D	ILE33
D	LEU34
E	PHE27
E	ARG28
E	ILE33
E	LEU34
F	PHE27

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site_id	SWS_FT_FI2
Number of Residues	3
Details	SITE: Key residue for inhibition of rat ASIC1a, probably binds to rat ASIC1a F-350 => ECO:0000269|PubMed:26680001

Chain	Residue	Details
D	LEU32
E	LEU32
F	LEU32

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site_id	SWS_FT_FI3
Number of Residues	1080
Details	TOPO_DOM: Extracellular => ECO:0000269|PubMed:34319232, ECO:0007744|PDB:7RNN

Chain	Residue	Details
A	TYR67-GLU427
B	TYR67-GLU427
C	TYR67-GLU427

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site_id	SWS_FT_FI4
Number of Residues	90
Details	TRANSMEM: Discontinuously helical => ECO:0000305|PubMed:34319232, ECO:0007744|PDB:7RNN

Chain	Residue	Details
A	ILE428-TYR458
B	ILE428-TYR458
C	ILE428-TYR458

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site_id	SWS_FT_FI5
Number of Residues	3
Details	SITE: Involved in channel desensitization; the process by which the channel becomes unresponsive to proton stimulation => ECO:0000250|UniProtKB:Q1XA76

Chain	Residue	Details
A	GLU79
B	GLU79
C	GLU79

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site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Involved in the inhibition by the spider venom psalmotoxin-1 => ECO:0000269|PubMed:19654327

Chain	Residue	Details
A	PHE352
B	PHE352
C	PHE352

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site_id	SWS_FT_FI7
Number of Residues	3
Details	SITE: Involved in proton-dependent gating => ECO:0000269|PubMed:19654327

Chain	Residue	Details
A	ASP357
B	ASP357
C	ASP357

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site_id	SWS_FT_FI8
Number of Residues	6
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:32915133, ECO:0000269|PubMed:34319232, ECO:0007744|PDB:7CFS, ECO:0007744|PDB:7CFT, ECO:0007744|PDB:7RNN

Chain	Residue	Details
A	ASN368
A	ASN395
B	ASN368
B	ASN395
C	ASN368
C	ASN395

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