6BSX

CRYSTAL STRUCTURE OF RHEB IN COMPLEX WITH COMPOUND 1 AT 1.65A RESOLUTION

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000287	molecular_function	magnesium ion binding
A	0003924	molecular_function	GTPase activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005681	cellular_component	spliceosomal complex
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005765	cellular_component	lysosomal membrane
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0007165	biological_process	signal transduction
A	0007264	biological_process	small GTPase-mediated signal transduction
A	0012505	cellular_component	endomembrane system
A	0014069	cellular_component	postsynaptic density
A	0016020	cellular_component	membrane
A	0016241	biological_process	regulation of macroautophagy
A	0016787	molecular_function	hydrolase activity
A	0019003	molecular_function	GDP binding
A	0019901	molecular_function	protein kinase binding
A	0030295	molecular_function	protein kinase activator activity
A	0031669	biological_process	cellular response to nutrient levels
A	0032006	biological_process	regulation of TOR signaling
A	0032008	biological_process	positive regulation of TOR signaling
A	0043539	molecular_function	protein serine/threonine kinase activator activity
A	0045202	cellular_component	synapse
A	0046872	molecular_function	metal ion binding
A	0048709	biological_process	oligodendrocyte differentiation
A	0048714	biological_process	positive regulation of oligodendrocyte differentiation
A	0051726	biological_process	regulation of cell cycle
A	0070062	cellular_component	extracellular exosome
A	0120163	biological_process	negative regulation of cold-induced thermogenesis
A	1904263	biological_process	positive regulation of TORC1 signaling
A	2000074	biological_process	regulation of type B pancreatic cell development
B	0000139	cellular_component	Golgi membrane
B	0000287	molecular_function	magnesium ion binding
B	0003924	molecular_function	GTPase activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005681	cellular_component	spliceosomal complex
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005765	cellular_component	lysosomal membrane
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007264	biological_process	small GTPase-mediated signal transduction
B	0012505	cellular_component	endomembrane system
B	0014069	cellular_component	postsynaptic density
B	0016020	cellular_component	membrane
B	0016241	biological_process	regulation of macroautophagy
B	0016787	molecular_function	hydrolase activity
B	0019003	molecular_function	GDP binding
B	0019901	molecular_function	protein kinase binding
B	0030295	molecular_function	protein kinase activator activity
B	0031669	biological_process	cellular response to nutrient levels
B	0032006	biological_process	regulation of TOR signaling
B	0032008	biological_process	positive regulation of TOR signaling
B	0043539	molecular_function	protein serine/threonine kinase activator activity
B	0045202	cellular_component	synapse
B	0046872	molecular_function	metal ion binding
B	0048709	biological_process	oligodendrocyte differentiation
B	0048714	biological_process	positive regulation of oligodendrocyte differentiation
B	0051726	biological_process	regulation of cell cycle
B	0070062	cellular_component	extracellular exosome
B	0120163	biological_process	negative regulation of cold-induced thermogenesis
B	1904263	biological_process	positive regulation of TORC1 signaling
B	2000074	biological_process	regulation of type B pancreatic cell development
C	0000139	cellular_component	Golgi membrane
C	0000287	molecular_function	magnesium ion binding
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005681	cellular_component	spliceosomal complex
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005765	cellular_component	lysosomal membrane
C	0005783	cellular_component	endoplasmic reticulum
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005794	cellular_component	Golgi apparatus
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007264	biological_process	small GTPase-mediated signal transduction
C	0012505	cellular_component	endomembrane system
C	0014069	cellular_component	postsynaptic density
C	0016020	cellular_component	membrane
C	0016241	biological_process	regulation of macroautophagy
C	0016787	molecular_function	hydrolase activity
C	0019003	molecular_function	GDP binding
C	0019901	molecular_function	protein kinase binding
C	0030295	molecular_function	protein kinase activator activity
C	0031669	biological_process	cellular response to nutrient levels
C	0032006	biological_process	regulation of TOR signaling
C	0032008	biological_process	positive regulation of TOR signaling
C	0043539	molecular_function	protein serine/threonine kinase activator activity
C	0045202	cellular_component	synapse
C	0046872	molecular_function	metal ion binding
C	0048709	biological_process	oligodendrocyte differentiation
C	0048714	biological_process	positive regulation of oligodendrocyte differentiation
C	0051726	biological_process	regulation of cell cycle
C	0070062	cellular_component	extracellular exosome
C	0120163	biological_process	negative regulation of cold-induced thermogenesis
C	1904263	biological_process	positive regulation of TORC1 signaling
C	2000074	biological_process	regulation of type B pancreatic cell development
D	0000139	cellular_component	Golgi membrane
D	0000287	molecular_function	magnesium ion binding
D	0003924	molecular_function	GTPase activity
D	0005515	molecular_function	protein binding
D	0005525	molecular_function	GTP binding
D	0005681	cellular_component	spliceosomal complex
D	0005737	cellular_component	cytoplasm
D	0005764	cellular_component	lysosome
D	0005765	cellular_component	lysosomal membrane
D	0005783	cellular_component	endoplasmic reticulum
D	0005789	cellular_component	endoplasmic reticulum membrane
D	0005794	cellular_component	Golgi apparatus
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007264	biological_process	small GTPase-mediated signal transduction
D	0012505	cellular_component	endomembrane system
D	0014069	cellular_component	postsynaptic density
D	0016020	cellular_component	membrane
D	0016241	biological_process	regulation of macroautophagy
D	0016787	molecular_function	hydrolase activity
D	0019003	molecular_function	GDP binding
D	0019901	molecular_function	protein kinase binding
D	0030295	molecular_function	protein kinase activator activity
D	0031669	biological_process	cellular response to nutrient levels
D	0032006	biological_process	regulation of TOR signaling
D	0032008	biological_process	positive regulation of TOR signaling
D	0043539	molecular_function	protein serine/threonine kinase activator activity
D	0045202	cellular_component	synapse
D	0046872	molecular_function	metal ion binding
D	0048709	biological_process	oligodendrocyte differentiation
D	0048714	biological_process	positive regulation of oligodendrocyte differentiation
D	0051726	biological_process	regulation of cell cycle
D	0070062	cellular_component	extracellular exosome
D	0120163	biological_process	negative regulation of cold-induced thermogenesis
D	1904263	biological_process	positive regulation of TORC1 signaling
D	2000074	biological_process	regulation of type B pancreatic cell development

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MG A 201

Chain	Residue
A	SER20
A	GDP203
A	HOH323
A	HOH337
A	HOH367
A	HOH371

---

site_id	AC2
Number of Residues	1
Details	binding site for residue ACT A 202

Chain	Residue
A	GLU28

---

site_id	AC3
Number of Residues	24
Details	binding site for residue GDP A 203

Chain	Residue
A	GLY18
A	LYS19
A	SER20
A	SER21
A	PHE31
A	ASN119
A	LYS120
A	ASP122
A	LEU123
A	SER149
A	ALA150
A	LYS151
A	MG201
A	HOH306
A	HOH311
A	HOH312
A	HOH323
A	HOH329
A	HOH337
A	HOH371
A	HOH391
A	HOH402
A	SER16
A	VAL17

---

site_id	AC4
Number of Residues	11
Details	binding site for residue E7S A 204

Chain	Residue
A	ALA10
A	GLU40
A	THR61
A	ALA62
A	GLY63
A	GLN64
A	TYR74
A	HOH303
A	HOH324
B	HIS55
B	HOH364

---

site_id	AC5
Number of Residues	4
Details	binding site for residue EDO A 205

Chain	Residue
A	ILE129
A	GLU133
A	HOH347
B	ASP158

---

site_id	AC6
Number of Residues	6
Details	binding site for residue MG B 201

Chain	Residue
B	SER20
B	GDP203
B	HOH316
B	HOH320
B	HOH365
B	HOH368

---

site_id	AC7
Number of Residues	2
Details	binding site for residue ACT B 202

Chain	Residue
B	GLU28
B	TYR35

---

site_id	AC8
Number of Residues	25
Details	binding site for residue GDP B 203

Chain	Residue
B	ARG15
B	SER16
B	VAL17
B	GLY18
B	LYS19
B	SER20
B	SER21
B	PHE31
B	ASP33
B	ASN119
B	LYS120
B	ASP122
B	LEU123
B	SER149
B	ALA150
B	MG201
B	HOH302
B	HOH305
B	HOH316
B	HOH320
B	HOH352
B	HOH356
B	HOH368
B	HOH386
B	HOH412

---

site_id	AC9
Number of Residues	11
Details	binding site for residue E7S B 204

Chain	Residue
A	HIS55
B	ALA10
B	GLU40
B	THR61
B	ALA62
B	GLY63
B	GLN64
B	TYR74
B	HOH304
B	HOH323
B	HOH362

---

site_id	AD1
Number of Residues	3
Details	binding site for residue EDO B 205

Chain	Residue
A	ASP158
A	HOH317
B	GLU133

---

site_id	AD2
Number of Residues	6
Details	binding site for residue MG C 201

Chain	Residue
C	SER20
C	GDP203
C	HOH319
C	HOH340
C	HOH349
C	HOH376

---

site_id	AD3
Number of Residues	2
Details	binding site for residue ACT C 202

Chain	Residue
C	GLU28
C	TYR35

---

site_id	AD4
Number of Residues	25
Details	binding site for residue GDP C 203

Chain	Residue
C	SER16
C	VAL17
C	GLY18
C	LYS19
C	SER20
C	SER21
C	PHE31
C	VAL32
C	ASP33
C	ASN119
C	LYS120
C	ASP122
C	LEU123
C	SER149
C	ALA150
C	LYS151
C	MG201
C	HOH314
C	HOH319
C	HOH322
C	HOH340
C	HOH362
C	HOH365
C	HOH373
C	HOH376

---

site_id	AD5
Number of Residues	11
Details	binding site for residue E7S C 204

Chain	Residue
C	ALA10
C	GLU40
C	THR61
C	ALA62
C	GLY63
C	GLN64
C	TYR74
C	HOH307
C	HOH335
C	HOH358
D	HIS55

---

site_id	AD6
Number of Residues	3
Details	binding site for residue EDO C 205

Chain	Residue
C	GLU133
C	HOH391
D	ASP158

---

site_id	AD7
Number of Residues	6
Details	binding site for residue MG D 201

Chain	Residue
D	SER20
D	GDP203
D	HOH328
D	HOH332
D	HOH351
D	HOH366

---

site_id	AD8
Number of Residues	2
Details	binding site for residue ACT D 202

Chain	Residue
D	GLU28
D	TYR35

---

site_id	AD9
Number of Residues	24
Details	binding site for residue GDP D 203

Chain	Residue
D	SER16
D	VAL17
D	GLY18
D	LYS19
D	SER20
D	SER21
D	PHE31
D	VAL32
D	ASP33
D	ASN119
D	LYS120
D	ASP122
D	LEU123
D	SER149
D	ALA150
D	LYS151
D	MG201
D	HOH324
D	HOH328
D	HOH330
D	HOH332
D	HOH346
D	HOH366
D	HOH376

---

site_id	AE1
Number of Residues	11
Details	binding site for residue E7S D 204

Chain	Residue
C	HIS55
D	ALA10
D	GLU40
D	THR61
D	ALA62
D	GLY63
D	GLN64
D	PHE70
D	TYR74
D	HOH301
D	HOH306

---

site_id	AE2
Number of Residues	4
Details	binding site for residue EDO D 205

Chain	Residue
C	ASP158
C	HOH347
D	GLU133
D	HOH388

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:1XTS, ECO:0007744|PDB:6BCU

Chain	Residue	Details
A	SER16
B	ASN119
B	ASP122
B	ALA150
C	SER16
C	GLY18
C	TYR35
C	ASN119
C	ASP122
C	ALA150
D	SER16
A	GLY18
D	GLY18
D	TYR35
D	ASN119
D	ASP122
D	ALA150
A	TYR35
A	ASN119
A	ASP122
A	ALA150
B	SER16
B	GLY18
B	TYR35

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:22819219, ECO:0000269|PubMed:32470140, ECO:0007744|PDB:1XTQ, ECO:0007744|PDB:3SEA, ECO:0007744|PDB:3T5G, ECO:0007744|PDB:7BTA, ECO:0007744|PDB:7BTC

Chain	Residue	Details
A	VAL17
B	VAL17
C	VAL17
D	VAL17

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:1XTS, ECO:0007744|PDB:6BCU

Chain	Residue	Details
A	LYS19
B	LYS19
C	LYS19
D	LYS19

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0000269|PubMed:29416044, ECO:0007744|PDB:5YXH, ECO:0007744|PDB:6BCU, ECO:0007744|PDB:6BSX, ECO:0007744|PDB:6BT0

Chain	Residue	Details
A	SER20
B	SER20
C	SER20
D	SER20

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0007744|PDB:1XTS

Chain	Residue	Details
A	SER21
A	VAL32
B	SER21
B	VAL32
C	SER21
C	VAL32
D	SER21
D	VAL32

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:22002721, ECO:0000269|PubMed:22819219, ECO:0000269|PubMed:29416044, ECO:0000269|PubMed:32470140, ECO:0007744|PDB:3SEA, ECO:0007744|PDB:6BT0, ECO:0007744|PDB:7BTA, ECO:0007744|PDB:7BTC

Chain	Residue	Details
A	ASP33
B	ASP33
C	ASP33
D	ASP33

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:15728574, ECO:0000269|PubMed:29236692, ECO:0007744|PDB:6BCU

Chain	Residue	Details
A	THR38
B	THR38
C	THR38
D	THR38

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	SITE: Important for autoinhibition of GTPase activity => ECO:0000269|PubMed:22819219

Chain	Residue	Details
A	TYR35
B	TYR35
C	TYR35
D	TYR35

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphoserine; by MAPKAPK5 => ECO:0000250|UniProtKB:Q921J2

Chain	Residue	Details
A	SER130
B	SER130
C	SER130
D	SER130

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:30514904

Chain	Residue	Details
A	LYS8
B	LYS8
C	LYS8
D	LYS8

---