6BSX

CRYSTAL STRUCTURE OF RHEB IN COMPLEX WITH COMPOUND 1 AT 1.65A RESOLUTION

Summary for 6BSX

• Entry DOI: 10.2210/pdb6bsx/pdb
• Related: 1XTQ
• Descriptor: GTP-binding protein Rheb, MAGNESIUM ION, ACETATE ION, ... (7 entities in total)
• Functional Keywords: mtorc1 g-protein, signaling protein
• Biological source: Homo sapiens (Human)
• Cellular location: Endomembrane system ; Lipid-anchor ; Cytoplasmic side : Q15382
• Total number of polymer chains: 4
• Total formula weight: 84003.51

Authors

Mahoney, S.J. (deposition date: 2017-12-04, release date: 2018-02-28, Last modification date: 2023-10-04)

Primary citation

Mahoney, S.J.,Narayan, S.,Molz, L.,Berstler, L.A.,Kang, S.A.,Vlasuk, G.P.,Saiah, E.
A small molecule inhibitor of Rheb selectively targets mTORC1 signaling.
Nat Commun, 9:548-548, 2018

PubMed Abstract: The small G-protein Rheb activates the mechanistic target of rapamycin complex 1 (mTORC1) in response to growth factor signals. mTORC1 is a master regulator of cellular growth and metabolism; aberrant mTORC1 signaling is associated with fibrotic, metabolic, and neurodegenerative diseases, cancers, and rare disorders. Point mutations in the Rheb switch II domain impair its ability to activate mTORC1. Here, we report the discovery of a small molecule (NR1) that binds Rheb in the switch II domain and selectively blocks mTORC1 signaling. NR1 potently inhibits mTORC1 driven phosphorylation of ribosomal protein S6 kinase beta-1 (S6K1) but does not inhibit phosphorylation of AKT or ERK. In contrast to rapamycin, NR1 does not cause inhibition of mTORC2 upon prolonged treatment. Furthermore, NR1 potently and selectively inhibits mTORC1 in mouse kidney and muscle in vivo. The data presented herein suggest that pharmacological inhibition of Rheb is an effective approach for selective inhibition of mTORC1 with therapeutic potential.

PubMed: 29416044
DOI: 10.1038/s41467-018-03035-z

Experimental method

X-RAY DIFFRACTION (1.65 Å)