5UGF
Crystal structure of human purine nucleoside phosphorylase (F159Y) mutant complexed with DADMe-ImmG and phosphate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000255 | biological_process | allantoin metabolic process |
| A | 0001882 | molecular_function | nucleoside binding |
| A | 0002060 | molecular_function | purine nucleobase binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| A | 0006148 | biological_process | inosine catabolic process |
| A | 0006149 | biological_process | deoxyinosine catabolic process |
| A | 0006157 | biological_process | deoxyadenosine catabolic process |
| A | 0006166 | biological_process | purine ribonucleoside salvage |
| A | 0006204 | biological_process | IMP catabolic process |
| A | 0006738 | biological_process | nicotinamide riboside catabolic process |
| A | 0006955 | biological_process | immune response |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0032743 | biological_process | positive regulation of interleukin-2 production |
| A | 0034418 | biological_process | urate biosynthetic process |
| A | 0034774 | cellular_component | secretory granule lumen |
| A | 0042102 | biological_process | positive regulation of T cell proliferation |
| A | 0042301 | molecular_function | phosphate ion binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043101 | biological_process | purine-containing compound salvage |
| A | 0046059 | biological_process | dAMP catabolic process |
| A | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| A | 0047975 | molecular_function | guanosine phosphorylase activity |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| B | 0000255 | biological_process | allantoin metabolic process |
| B | 0001882 | molecular_function | nucleoside binding |
| B | 0002060 | molecular_function | purine nucleobase binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| B | 0006148 | biological_process | inosine catabolic process |
| B | 0006149 | biological_process | deoxyinosine catabolic process |
| B | 0006157 | biological_process | deoxyadenosine catabolic process |
| B | 0006166 | biological_process | purine ribonucleoside salvage |
| B | 0006204 | biological_process | IMP catabolic process |
| B | 0006738 | biological_process | nicotinamide riboside catabolic process |
| B | 0006955 | biological_process | immune response |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0032743 | biological_process | positive regulation of interleukin-2 production |
| B | 0034418 | biological_process | urate biosynthetic process |
| B | 0034774 | cellular_component | secretory granule lumen |
| B | 0042102 | biological_process | positive regulation of T cell proliferation |
| B | 0042301 | molecular_function | phosphate ion binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0043101 | biological_process | purine-containing compound salvage |
| B | 0046059 | biological_process | dAMP catabolic process |
| B | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| B | 0047975 | molecular_function | guanosine phosphorylase activity |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| C | 0000255 | biological_process | allantoin metabolic process |
| C | 0001882 | molecular_function | nucleoside binding |
| C | 0002060 | molecular_function | purine nucleobase binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005576 | cellular_component | extracellular region |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| C | 0006148 | biological_process | inosine catabolic process |
| C | 0006149 | biological_process | deoxyinosine catabolic process |
| C | 0006157 | biological_process | deoxyadenosine catabolic process |
| C | 0006166 | biological_process | purine ribonucleoside salvage |
| C | 0006204 | biological_process | IMP catabolic process |
| C | 0006738 | biological_process | nicotinamide riboside catabolic process |
| C | 0006955 | biological_process | immune response |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0009165 | biological_process | nucleotide biosynthetic process |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0032743 | biological_process | positive regulation of interleukin-2 production |
| C | 0034418 | biological_process | urate biosynthetic process |
| C | 0034774 | cellular_component | secretory granule lumen |
| C | 0042102 | biological_process | positive regulation of T cell proliferation |
| C | 0042301 | molecular_function | phosphate ion binding |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0043101 | biological_process | purine-containing compound salvage |
| C | 0046059 | biological_process | dAMP catabolic process |
| C | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| C | 0047975 | molecular_function | guanosine phosphorylase activity |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| D | 0000255 | biological_process | allantoin metabolic process |
| D | 0001882 | molecular_function | nucleoside binding |
| D | 0002060 | molecular_function | purine nucleobase binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005576 | cellular_component | extracellular region |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| D | 0006148 | biological_process | inosine catabolic process |
| D | 0006149 | biological_process | deoxyinosine catabolic process |
| D | 0006157 | biological_process | deoxyadenosine catabolic process |
| D | 0006166 | biological_process | purine ribonucleoside salvage |
| D | 0006204 | biological_process | IMP catabolic process |
| D | 0006738 | biological_process | nicotinamide riboside catabolic process |
| D | 0006955 | biological_process | immune response |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0009165 | biological_process | nucleotide biosynthetic process |
| D | 0009410 | biological_process | response to xenobiotic stimulus |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0032743 | biological_process | positive regulation of interleukin-2 production |
| D | 0034418 | biological_process | urate biosynthetic process |
| D | 0034774 | cellular_component | secretory granule lumen |
| D | 0042102 | biological_process | positive regulation of T cell proliferation |
| D | 0042301 | molecular_function | phosphate ion binding |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0043101 | biological_process | purine-containing compound salvage |
| D | 0046059 | biological_process | dAMP catabolic process |
| D | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| D | 0047975 | molecular_function | guanosine phosphorylase activity |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| E | 0000255 | biological_process | allantoin metabolic process |
| E | 0001882 | molecular_function | nucleoside binding |
| E | 0002060 | molecular_function | purine nucleobase binding |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005576 | cellular_component | extracellular region |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| E | 0006148 | biological_process | inosine catabolic process |
| E | 0006149 | biological_process | deoxyinosine catabolic process |
| E | 0006157 | biological_process | deoxyadenosine catabolic process |
| E | 0006166 | biological_process | purine ribonucleoside salvage |
| E | 0006204 | biological_process | IMP catabolic process |
| E | 0006738 | biological_process | nicotinamide riboside catabolic process |
| E | 0006955 | biological_process | immune response |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0009165 | biological_process | nucleotide biosynthetic process |
| E | 0009410 | biological_process | response to xenobiotic stimulus |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0032743 | biological_process | positive regulation of interleukin-2 production |
| E | 0034418 | biological_process | urate biosynthetic process |
| E | 0034774 | cellular_component | secretory granule lumen |
| E | 0042102 | biological_process | positive regulation of T cell proliferation |
| E | 0042301 | molecular_function | phosphate ion binding |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0043101 | biological_process | purine-containing compound salvage |
| E | 0046059 | biological_process | dAMP catabolic process |
| E | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| E | 0047975 | molecular_function | guanosine phosphorylase activity |
| E | 0070062 | cellular_component | extracellular exosome |
| E | 1904813 | cellular_component | ficolin-1-rich granule lumen |
| F | 0000255 | biological_process | allantoin metabolic process |
| F | 0001882 | molecular_function | nucleoside binding |
| F | 0002060 | molecular_function | purine nucleobase binding |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005576 | cellular_component | extracellular region |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| F | 0006148 | biological_process | inosine catabolic process |
| F | 0006149 | biological_process | deoxyinosine catabolic process |
| F | 0006157 | biological_process | deoxyadenosine catabolic process |
| F | 0006166 | biological_process | purine ribonucleoside salvage |
| F | 0006204 | biological_process | IMP catabolic process |
| F | 0006738 | biological_process | nicotinamide riboside catabolic process |
| F | 0006955 | biological_process | immune response |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0009165 | biological_process | nucleotide biosynthetic process |
| F | 0009410 | biological_process | response to xenobiotic stimulus |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0032743 | biological_process | positive regulation of interleukin-2 production |
| F | 0034418 | biological_process | urate biosynthetic process |
| F | 0034774 | cellular_component | secretory granule lumen |
| F | 0042102 | biological_process | positive regulation of T cell proliferation |
| F | 0042301 | molecular_function | phosphate ion binding |
| F | 0042802 | molecular_function | identical protein binding |
| F | 0043101 | biological_process | purine-containing compound salvage |
| F | 0046059 | biological_process | dAMP catabolic process |
| F | 0046638 | biological_process | positive regulation of alpha-beta T cell differentiation |
| F | 0047975 | molecular_function | guanosine phosphorylase activity |
| F | 0070062 | cellular_component | extracellular exosome |
| F | 1904813 | cellular_component | ficolin-1-rich granule lumen |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | binding site for residue PO4 A 301 |
| Chain | Residue |
| A | GLY32 |
| A | HOH411 |
| A | HOH413 |
| A | SER33 |
| A | HIS64 |
| A | ARG84 |
| A | HIS86 |
| A | ASN115 |
| A | ALA116 |
| A | SER220 |
| A | IM5308 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 302 |
| Chain | Residue |
| A | ARG148 |
| C | PRO92 |
| C | GLN144 |
| C | HOH452 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 303 |
| Chain | Residue |
| A | ASN151 |
| A | ASP152 |
| A | GLU153 |
| A | ARG154 |
| A | HOH403 |
| A | HOH471 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 304 |
| Chain | Residue |
| A | ARG133 |
| A | HOH412 |
| C | ARG207 |
| C | TYR249 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 305 |
| Chain | Residue |
| A | ARG207 |
| A | TYR249 |
| A | HOH420 |
| B | ARG133 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 A 306 |
| Chain | Residue |
| A | ARG133 |
| A | ARG168 |
| A | ARG171 |
| A | GLU189 |
| C | TYR249 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 307 |
| Chain | Residue |
| A | ARG229 |
| A | HOH410 |
| A | HOH426 |
| A | HOH456 |
| site_id | AC8 |
| Number of Residues | 15 |
| Details | binding site for residue IM5 A 308 |
| Chain | Residue |
| A | TYR88 |
| A | ALA116 |
| A | GLY118 |
| A | VAL195 |
| A | PHE200 |
| A | GLU201 |
| A | VAL217 |
| A | GLY218 |
| A | MET219 |
| A | THR242 |
| A | ASN243 |
| A | HIS257 |
| A | PO4301 |
| A | HOH460 |
| B | TYR159 |
| site_id | AC9 |
| Number of Residues | 11 |
| Details | binding site for residue PO4 B 301 |
| Chain | Residue |
| B | GLY32 |
| B | SER33 |
| B | HIS64 |
| B | ARG84 |
| B | HIS86 |
| B | ASN115 |
| B | ALA116 |
| B | SER220 |
| B | IM5306 |
| B | HOH412 |
| B | HOH416 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 B 302 |
| Chain | Residue |
| A | PRO92 |
| A | GLN144 |
| B | MET1 |
| B | ARG148 |
| site_id | AD2 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 B 303 |
| Chain | Residue |
| B | ARG207 |
| B | HOH405 |
| C | ARG133 |
| site_id | AD3 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 B 304 |
| Chain | Residue |
| B | THR43 |
| B | GLN44 |
| B | HOH476 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 B 305 |
| Chain | Residue |
| B | THR6 |
| B | TYR7 |
| B | GLU8 |
| B | ARG154 |
| site_id | AD5 |
| Number of Residues | 17 |
| Details | binding site for residue IM5 B 306 |
| Chain | Residue |
| B | TYR88 |
| B | ALA116 |
| B | GLY118 |
| B | VAL195 |
| B | PHE200 |
| B | GLU201 |
| B | VAL217 |
| B | GLY218 |
| B | MET219 |
| B | THR242 |
| B | ASN243 |
| B | HIS257 |
| B | VAL260 |
| B | PO4301 |
| B | HOH416 |
| B | HOH452 |
| C | TYR159 |
| site_id | AD6 |
| Number of Residues | 11 |
| Details | binding site for residue PO4 C 301 |
| Chain | Residue |
| C | HIS86 |
| C | ASN115 |
| C | ALA116 |
| C | SER220 |
| C | IM5305 |
| C | HOH407 |
| C | HOH411 |
| C | GLY32 |
| C | SER33 |
| C | HIS64 |
| C | ARG84 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 C 302 |
| Chain | Residue |
| B | PRO92 |
| B | GLN144 |
| C | ARG148 |
| C | HOH444 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 C 303 |
| Chain | Residue |
| B | TYR249 |
| C | ARG133 |
| C | ARG168 |
| C | ARG171 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 C 304 |
| Chain | Residue |
| C | THR6 |
| C | TYR7 |
| C | GLU8 |
| C | ARG154 |
| site_id | AE1 |
| Number of Residues | 15 |
| Details | binding site for residue IM5 C 305 |
| Chain | Residue |
| A | TYR159 |
| C | TYR88 |
| C | ALA116 |
| C | GLY118 |
| C | VAL195 |
| C | PHE200 |
| C | GLU201 |
| C | VAL217 |
| C | GLY218 |
| C | MET219 |
| C | THR242 |
| C | ASN243 |
| C | HIS257 |
| C | PO4301 |
| C | HOH438 |
| site_id | AE2 |
| Number of Residues | 10 |
| Details | binding site for residue PO4 D 301 |
| Chain | Residue |
| D | GLY32 |
| D | SER33 |
| D | HIS64 |
| D | ARG84 |
| D | HIS86 |
| D | ASN115 |
| D | ALA116 |
| D | SER220 |
| D | IM5307 |
| D | HOH410 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 D 302 |
| Chain | Residue |
| D | ARG148 |
| F | PRO92 |
| F | GLN144 |
| F | HOH413 |
| site_id | AE4 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 D 303 |
| Chain | Residue |
| D | PRO92 |
| D | GLN144 |
| D | HOH420 |
| E | ARG148 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 D 304 |
| Chain | Residue |
| D | ARG133 |
| D | HOH402 |
| F | ARG207 |
| F | TYR249 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 D 305 |
| Chain | Residue |
| D | ARG207 |
| D | TYR249 |
| D | HOH424 |
| E | ARG133 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 D 306 |
| Chain | Residue |
| D | ARG133 |
| D | ARG168 |
| D | ARG171 |
| site_id | AE8 |
| Number of Residues | 17 |
| Details | binding site for residue IM5 D 307 |
| Chain | Residue |
| D | TYR88 |
| D | ALA116 |
| D | ALA117 |
| D | GLY118 |
| D | VAL195 |
| D | PHE200 |
| D | GLU201 |
| D | VAL217 |
| D | GLY218 |
| D | MET219 |
| D | THR242 |
| D | ASN243 |
| D | HIS257 |
| D | VAL260 |
| D | PO4301 |
| D | HOH432 |
| E | TYR159 |
| site_id | AE9 |
| Number of Residues | 11 |
| Details | binding site for residue PO4 E 301 |
| Chain | Residue |
| E | GLY32 |
| E | SER33 |
| E | HIS64 |
| E | ARG84 |
| E | HIS86 |
| E | ASN115 |
| E | ALA116 |
| E | SER220 |
| E | IM5306 |
| E | HOH401 |
| E | HOH433 |
| site_id | AF1 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 E 302 |
| Chain | Residue |
| E | PRO92 |
| E | GLN144 |
| E | HOH402 |
| F | ARG148 |
| site_id | AF2 |
| Number of Residues | 1 |
| Details | binding site for residue PO4 E 303 |
| Chain | Residue |
| E | GLN44 |
| site_id | AF3 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 E 304 |
| Chain | Residue |
| E | ARG133 |
| E | ARG168 |
| E | ARG171 |
| E | HOH419 |
| site_id | AF4 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 E 305 |
| Chain | Residue |
| E | ARG207 |
| E | HOH415 |
| F | ARG133 |
| site_id | AF5 |
| Number of Residues | 15 |
| Details | binding site for residue IM5 E 306 |
| Chain | Residue |
| E | TYR88 |
| E | ALA116 |
| E | GLY118 |
| E | PHE200 |
| E | GLU201 |
| E | VAL217 |
| E | GLY218 |
| E | MET219 |
| E | THR242 |
| E | ASN243 |
| E | HIS257 |
| E | VAL260 |
| E | PO4301 |
| E | HOH427 |
| F | TYR159 |
| site_id | AF6 |
| Number of Residues | 11 |
| Details | binding site for residue PO4 F 301 |
| Chain | Residue |
| F | GLY32 |
| F | SER33 |
| F | HIS64 |
| F | ARG84 |
| F | HIS86 |
| F | ASN115 |
| F | ALA116 |
| F | SER220 |
| F | IM5303 |
| F | HOH411 |
| F | HOH415 |
| site_id | AF7 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 F 302 |
| Chain | Residue |
| E | TYR249 |
| F | ARG133 |
| F | ARG168 |
| F | ARG171 |
| site_id | AF8 |
| Number of Residues | 17 |
| Details | binding site for residue IM5 F 303 |
| Chain | Residue |
| D | TYR159 |
| F | TYR88 |
| F | ALA116 |
| F | ALA117 |
| F | GLY118 |
| F | PHE200 |
| F | GLU201 |
| F | VAL217 |
| F | GLY218 |
| F | MET219 |
| F | THR242 |
| F | ASN243 |
| F | HIS257 |
| F | VAL260 |
| F | PO4301 |
| F | HOH415 |
| F | HOH440 |
Functional Information from PROSITE/UniProt
| site_id | PS01240 |
| Number of Residues | 42 |
| Details | PNP_MTAP_2 Purine and other phosphorylases family 2 signature. VmmqGrfHmYegypLwkvTfpVrVfhllGvdt.LVvtNAaGGL |
| Chain | Residue | Details |
| A | VAL79-LEU120 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:23438750, ECO:0000305|PubMed:14706628, ECO:0000305|PubMed:1763067, ECO:0007744|PDB:1RCT, ECO:0007744|PDB:1ULA, ECO:0007744|PDB:1ULB, ECO:0007744|PDB:4EAR, ECO:0007744|PDB:4EB8, ECO:0007744|PDB:4GKA |
| Chain | Residue | Details |
| A | SER33 | |
| B | SER33 | |
| C | SER33 | |
| D | SER33 | |
| E | SER33 | |
| F | SER33 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P55859 |
| Chain | Residue | Details |
| A | HIS64 | |
| B | HIS64 | |
| C | HIS64 | |
| D | HIS64 | |
| E | HIS64 | |
| F | HIS64 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:23438750, ECO:0000305|PubMed:14706628, ECO:0000305|PubMed:1763067, ECO:0007744|PDB:1RCT, ECO:0007744|PDB:1ULA, ECO:0007744|PDB:1ULB, ECO:0007744|PDB:1V3Q, ECO:0007744|PDB:4EAR, ECO:0007744|PDB:4EB8, ECO:0007744|PDB:4GKA |
| Chain | Residue | Details |
| A | ARG84 | |
| B | ARG84 | |
| C | ARG84 | |
| D | ARG84 | |
| E | ARG84 | |
| F | ARG84 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14706628, ECO:0000269|PubMed:23438750, ECO:0007744|PDB:1RCT, ECO:0007744|PDB:4EAR, ECO:0007744|PDB:4EB8 |
| Chain | Residue | Details |
| A | TYR88 | |
| B | TYR88 | |
| C | TYR88 | |
| D | TYR88 | |
| E | TYR88 | |
| F | TYR88 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14706628, ECO:0000269|PubMed:23438750, ECO:0000305|PubMed:1763067, ECO:0007744|PDB:1RCT, ECO:0007744|PDB:1ULB, ECO:0007744|PDB:4EAR, ECO:0007744|PDB:4EB8, ECO:0007744|PDB:4GKA |
| Chain | Residue | Details |
| A | ALA116 | |
| C | GLU201 | |
| C | SER220 | |
| C | ASN243 | |
| D | ALA116 | |
| D | GLU201 | |
| D | SER220 | |
| D | ASN243 | |
| E | ALA116 | |
| E | GLU201 | |
| E | SER220 | |
| A | GLU201 | |
| E | ASN243 | |
| F | ALA116 | |
| F | GLU201 | |
| F | SER220 | |
| F | ASN243 | |
| A | SER220 | |
| A | ASN243 | |
| B | ALA116 | |
| B | GLU201 | |
| B | SER220 | |
| B | ASN243 | |
| C | ALA116 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:14706628, ECO:0007744|PDB:1RCT |
| Chain | Residue | Details |
| A | MET219 | |
| E | HIS257 | |
| F | MET219 | |
| F | HIS257 | |
| A | HIS257 | |
| B | MET219 | |
| B | HIS257 | |
| C | MET219 | |
| C | HIS257 | |
| D | MET219 | |
| D | HIS257 | |
| E | MET219 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | SITE: Important for substrate specificity => ECO:0000269|PubMed:9305964 |
| Chain | Residue | Details |
| A | ASN243 | |
| B | ASN243 | |
| C | ASN243 | |
| D | ASN243 | |
| E | ASN243 | |
| F | ASN243 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 6 |
| Details | MOD_RES: N-acetylmethionine => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895 |
| Chain | Residue | Details |
| A | MET1 | |
| B | MET1 | |
| C | MET1 | |
| D | MET1 | |
| E | MET1 | |
| F | MET1 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER251 | |
| B | SER251 | |
| C | SER251 | |
| D | SER251 | |
| E | SER251 | |
| F | SER251 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| A | SER33 | hydrogen bond donor |
| A | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| A | HIS64 | electrostatic stabiliser |
| A | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| A | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| A | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| A | MET219 | electrostatic stabiliser, hydrogen bond donor |
| A | SER220 | electrostatic stabiliser, hydrogen bond donor |
| A | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
| site_id | MCSA2 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| B | SER33 | hydrogen bond donor |
| B | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| B | HIS64 | electrostatic stabiliser |
| B | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| B | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| B | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| B | MET219 | electrostatic stabiliser, hydrogen bond donor |
| B | SER220 | electrostatic stabiliser, hydrogen bond donor |
| B | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
| site_id | MCSA3 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| C | SER33 | hydrogen bond donor |
| C | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| C | HIS64 | electrostatic stabiliser |
| C | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| C | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| C | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| C | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| C | MET219 | electrostatic stabiliser, hydrogen bond donor |
| C | SER220 | electrostatic stabiliser, hydrogen bond donor |
| C | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
| site_id | MCSA4 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| D | SER33 | hydrogen bond donor |
| D | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| D | HIS64 | electrostatic stabiliser |
| D | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| D | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| D | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| D | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| D | MET219 | electrostatic stabiliser, hydrogen bond donor |
| D | SER220 | electrostatic stabiliser, hydrogen bond donor |
| D | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
| site_id | MCSA5 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| E | SER33 | hydrogen bond donor |
| E | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| E | HIS64 | electrostatic stabiliser |
| E | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| E | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| E | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| E | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| E | MET219 | electrostatic stabiliser, hydrogen bond donor |
| E | SER220 | electrostatic stabiliser, hydrogen bond donor |
| E | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
| site_id | MCSA6 |
| Number of Residues | 10 |
| Details | M-CSA 17 |
| Chain | Residue | Details |
| F | SER33 | hydrogen bond donor |
| F | HIS257 | electrostatic stabiliser, hydrogen bond acceptor |
| F | HIS64 | electrostatic stabiliser |
| F | HIS86 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| F | TYR88 | electrostatic stabiliser, hydrogen bond donor |
| F | GLU89 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| F | ALA116 | electrostatic stabiliser, hydrogen bond donor |
| F | MET219 | electrostatic stabiliser, hydrogen bond donor |
| F | SER220 | electrostatic stabiliser, hydrogen bond donor |
| F | ASN243 | electrostatic stabiliser, hydrogen bond donor, polar interaction |
