4CDG

Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003676	molecular_function	nucleic acid binding
A	0004386	molecular_function	helicase activity
A	0005524	molecular_function	ATP binding
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0006310	biological_process	DNA recombination
A	0043138	molecular_function	3'-5' DNA helicase activity
B	0000166	molecular_function	nucleotide binding
B	0003676	molecular_function	nucleic acid binding
B	0004386	molecular_function	helicase activity
B	0005524	molecular_function	ATP binding
B	0006260	biological_process	DNA replication
B	0006281	biological_process	DNA repair
B	0006310	biological_process	DNA recombination
B	0043138	molecular_function	3'-5' DNA helicase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ADP A 1634

Chain	Residue
A	LEU665
A	ARG982
A	ASN1242
A	ASN667
A	ARG669
A	GLN672
A	GLY692
A	GLY693
A	GLY694
A	LYS695
A	SER696

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 1643

Chain	Residue
A	CYS1036
A	CYS1055
A	CYS1063
A	CYS1066

---

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE ADP B 1634

Chain	Residue
B	LEU665
B	ASN667
B	ARG669
B	GLN672
B	PRO690
B	GLY692
B	GLY693
B	GLY694
B	LYS695
B	SER696
B	ARG982
B	ASN1242

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1643

Chain	Residue
B	CYS1036
B	CYS1055
B	CYS1063
B	CYS1066

---

Functional Information from PROSITE/UniProt

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ArFVIDEAHC

Chain	Residue	Details
A	ALA790-CYS799

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	350
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	Region: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	28
Details	Region: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	34
Details	Region: {"description":"Necessary for ssDNA and DNA Holliday junction binding","evidences":[{"source":"PubMed","id":"20639533","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Motif: {"description":"DEAH box"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CDG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CDG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Site: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Site: {"description":"3' overhang DNA-binding; via amide nitrogen","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Site: {"description":"3' overhang DNA-binding","evidences":[{"source":"PubMed","id":"24816114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25901030","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4CGZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4O3M","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	7
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---