4CDG

Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003676	molecular_function	nucleic acid binding
A	0004386	molecular_function	helicase activity
A	0005524	molecular_function	ATP binding
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0006310	biological_process	DNA recombination
A	0043138	molecular_function	3'-5' DNA helicase activity
A	0044237	biological_process	cellular metabolic process
B	0000166	molecular_function	nucleotide binding
B	0003676	molecular_function	nucleic acid binding
B	0004386	molecular_function	helicase activity
B	0005524	molecular_function	ATP binding
B	0006260	biological_process	DNA replication
B	0006281	biological_process	DNA repair
B	0006310	biological_process	DNA recombination
B	0043138	molecular_function	3'-5' DNA helicase activity
B	0044237	biological_process	cellular metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ADP A 1634

Chain	Residue
A	LEU665
A	ARG982
A	ASN1242
A	ASN667
A	ARG669
A	GLN672
A	GLY692
A	GLY693
A	GLY694
A	LYS695
A	SER696

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 1643

Chain	Residue
A	CYS1036
A	CYS1055
A	CYS1063
A	CYS1066

---

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE ADP B 1634

Chain	Residue
B	LEU665
B	ASN667
B	ARG669
B	GLN672
B	PRO690
B	GLY692
B	GLY693
B	GLY694
B	LYS695
B	SER696
B	ARG982
B	ASN1242

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 1643

Chain	Residue
B	CYS1036
B	CYS1055
B	CYS1063
B	CYS1066

---

Functional Information from PROSITE/UniProt

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. ArFVIDEAHC

Chain	Residue	Details
A	ALA790-CYS799

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CDG, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M

Chain	Residue	Details
A	PHE668
B	CYS1055
B	CYS1063
B	CYS1066
A	GLY692
A	CYS1036
A	CYS1055
A	CYS1063
A	CYS1066
B	PHE668
B	GLY692
B	CYS1036

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CDG, ECO:0007744|PDB:4CGZ

Chain	Residue	Details
A	ARG982
A	ASN1242
B	ARG982
B	ASN1242

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: 3' overhang DNA-binding => ECO:0000269|PubMed:25901030

Chain	Residue	Details
A	ARG717
A	ARG808
B	ARG717
B	ARG808

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	SITE: 3' overhang DNA-binding; via amide nitrogen => ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M

Chain	Residue	Details
A	ALA920
B	ALA920

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: 3' overhang DNA-binding => ECO:0000269|PubMed:24816114, ECO:0000269|PubMed:25901030, ECO:0007744|PDB:4CGZ, ECO:0007744|PDB:4O3M

Chain	Residue	Details
A	THR946
A	LYS968
A	THR1110
B	THR946
B	LYS968
B	THR1110

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS863
B	LYS863

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER1197
B	SER1197

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
A	SER1295
A	SER1296
B	SER1295
B	SER1296

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS1125
A	LYS1199
A	LYS1207
B	LYS1125
B	LYS1199
B	LYS1207

---