4CDG
Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.666, 88.425, 95.120 |
| Unit cell angles | 69.39, 89.84, 77.07 |
Refinement procedure
| Resolution | 42.881 - 2.794 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wwy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.850 |
| Number of reflections | 43258 | |
| <I/σ(I)> | 15.9 | 2.2 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 7.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MES PH 6.0, 22% PEG 20K |
