Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3PHB

Crystal Structure of human purine nucleoside phosphorylase in complex with DADMe-ImmG

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0000255	biological_process	allantoin metabolic process
E	0001882	molecular_function	nucleoside binding
E	0002060	molecular_function	purine nucleobase binding
E	0003824	molecular_function	catalytic activity
E	0004731	molecular_function	purine-nucleoside phosphorylase activity
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006139	biological_process	nucleobase-containing compound metabolic process
E	0006148	biological_process	inosine catabolic process
E	0006149	biological_process	deoxyinosine catabolic process
E	0006157	biological_process	deoxyadenosine catabolic process
E	0006166	biological_process	purine ribonucleoside salvage
E	0006204	biological_process	IMP catabolic process
E	0006738	biological_process	nicotinamide riboside catabolic process
E	0006955	biological_process	immune response
E	0009116	biological_process	nucleoside metabolic process
E	0009165	biological_process	nucleotide biosynthetic process
E	0009410	biological_process	response to xenobiotic stimulus
E	0016757	molecular_function	glycosyltransferase activity
E	0016763	molecular_function	pentosyltransferase activity
E	0032743	biological_process	positive regulation of interleukin-2 production
E	0034418	biological_process	urate biosynthetic process
E	0034774	cellular_component	secretory granule lumen
E	0042102	biological_process	positive regulation of T cell proliferation
E	0042301	molecular_function	phosphate ion binding
E	0042802	molecular_function	identical protein binding
E	0043101	biological_process	purine-containing compound salvage
E	0046059	biological_process	dAMP catabolic process
E	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
E	0047975	molecular_function	guanosine phosphorylase activity
E	0070062	cellular_component	extracellular exosome
E	1904813	cellular_component	ficolin-1-rich granule lumen
Q	0000255	biological_process	allantoin metabolic process
Q	0001882	molecular_function	nucleoside binding
Q	0002060	molecular_function	purine nucleobase binding
Q	0003824	molecular_function	catalytic activity
Q	0004731	molecular_function	purine-nucleoside phosphorylase activity
Q	0005515	molecular_function	protein binding
Q	0005576	cellular_component	extracellular region
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006139	biological_process	nucleobase-containing compound metabolic process
Q	0006148	biological_process	inosine catabolic process
Q	0006149	biological_process	deoxyinosine catabolic process
Q	0006157	biological_process	deoxyadenosine catabolic process
Q	0006166	biological_process	purine ribonucleoside salvage
Q	0006204	biological_process	IMP catabolic process
Q	0006738	biological_process	nicotinamide riboside catabolic process
Q	0006955	biological_process	immune response
Q	0009116	biological_process	nucleoside metabolic process
Q	0009165	biological_process	nucleotide biosynthetic process
Q	0009410	biological_process	response to xenobiotic stimulus
Q	0016757	molecular_function	glycosyltransferase activity
Q	0016763	molecular_function	pentosyltransferase activity
Q	0032743	biological_process	positive regulation of interleukin-2 production
Q	0034418	biological_process	urate biosynthetic process
Q	0034774	cellular_component	secretory granule lumen
Q	0042102	biological_process	positive regulation of T cell proliferation
Q	0042301	molecular_function	phosphate ion binding
Q	0042802	molecular_function	identical protein binding
Q	0043101	biological_process	purine-containing compound salvage
Q	0046059	biological_process	dAMP catabolic process
Q	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
Q	0047975	molecular_function	guanosine phosphorylase activity
Q	0070062	cellular_component	extracellular exosome
Q	1904813	cellular_component	ficolin-1-rich granule lumen
S	0000255	biological_process	allantoin metabolic process
S	0001882	molecular_function	nucleoside binding
S	0002060	molecular_function	purine nucleobase binding
S	0003824	molecular_function	catalytic activity
S	0004731	molecular_function	purine-nucleoside phosphorylase activity
S	0005515	molecular_function	protein binding
S	0005576	cellular_component	extracellular region
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0006139	biological_process	nucleobase-containing compound metabolic process
S	0006148	biological_process	inosine catabolic process
S	0006149	biological_process	deoxyinosine catabolic process
S	0006157	biological_process	deoxyadenosine catabolic process
S	0006166	biological_process	purine ribonucleoside salvage
S	0006204	biological_process	IMP catabolic process
S	0006738	biological_process	nicotinamide riboside catabolic process
S	0006955	biological_process	immune response
S	0009116	biological_process	nucleoside metabolic process
S	0009165	biological_process	nucleotide biosynthetic process
S	0009410	biological_process	response to xenobiotic stimulus
S	0016757	molecular_function	glycosyltransferase activity
S	0016763	molecular_function	pentosyltransferase activity
S	0032743	biological_process	positive regulation of interleukin-2 production
S	0034418	biological_process	urate biosynthetic process
S	0034774	cellular_component	secretory granule lumen
S	0042102	biological_process	positive regulation of T cell proliferation
S	0042301	molecular_function	phosphate ion binding
S	0042802	molecular_function	identical protein binding
S	0043101	biological_process	purine-containing compound salvage
S	0046059	biological_process	dAMP catabolic process
S	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
S	0047975	molecular_function	guanosine phosphorylase activity
S	0070062	cellular_component	extracellular exosome
S	1904813	cellular_component	ficolin-1-rich granule lumen
T	0000255	biological_process	allantoin metabolic process
T	0001882	molecular_function	nucleoside binding
T	0002060	molecular_function	purine nucleobase binding
T	0003824	molecular_function	catalytic activity
T	0004731	molecular_function	purine-nucleoside phosphorylase activity
T	0005515	molecular_function	protein binding
T	0005576	cellular_component	extracellular region
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006139	biological_process	nucleobase-containing compound metabolic process
T	0006148	biological_process	inosine catabolic process
T	0006149	biological_process	deoxyinosine catabolic process
T	0006157	biological_process	deoxyadenosine catabolic process
T	0006166	biological_process	purine ribonucleoside salvage
T	0006204	biological_process	IMP catabolic process
T	0006738	biological_process	nicotinamide riboside catabolic process
T	0006955	biological_process	immune response
T	0009116	biological_process	nucleoside metabolic process
T	0009165	biological_process	nucleotide biosynthetic process
T	0009410	biological_process	response to xenobiotic stimulus
T	0016757	molecular_function	glycosyltransferase activity
T	0016763	molecular_function	pentosyltransferase activity
T	0032743	biological_process	positive regulation of interleukin-2 production
T	0034418	biological_process	urate biosynthetic process
T	0034774	cellular_component	secretory granule lumen
T	0042102	biological_process	positive regulation of T cell proliferation
T	0042301	molecular_function	phosphate ion binding
T	0042802	molecular_function	identical protein binding
T	0043101	biological_process	purine-containing compound salvage
T	0046059	biological_process	dAMP catabolic process
T	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
T	0047975	molecular_function	guanosine phosphorylase activity
T	0070062	cellular_component	extracellular exosome
T	1904813	cellular_component	ficolin-1-rich granule lumen
U	0000255	biological_process	allantoin metabolic process
U	0001882	molecular_function	nucleoside binding
U	0002060	molecular_function	purine nucleobase binding
U	0003824	molecular_function	catalytic activity
U	0004731	molecular_function	purine-nucleoside phosphorylase activity
U	0005515	molecular_function	protein binding
U	0005576	cellular_component	extracellular region
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0006139	biological_process	nucleobase-containing compound metabolic process
U	0006148	biological_process	inosine catabolic process
U	0006149	biological_process	deoxyinosine catabolic process
U	0006157	biological_process	deoxyadenosine catabolic process
U	0006166	biological_process	purine ribonucleoside salvage
U	0006204	biological_process	IMP catabolic process
U	0006738	biological_process	nicotinamide riboside catabolic process
U	0006955	biological_process	immune response
U	0009116	biological_process	nucleoside metabolic process
U	0009165	biological_process	nucleotide biosynthetic process
U	0009410	biological_process	response to xenobiotic stimulus
U	0016757	molecular_function	glycosyltransferase activity
U	0016763	molecular_function	pentosyltransferase activity
U	0032743	biological_process	positive regulation of interleukin-2 production
U	0034418	biological_process	urate biosynthetic process
U	0034774	cellular_component	secretory granule lumen
U	0042102	biological_process	positive regulation of T cell proliferation
U	0042301	molecular_function	phosphate ion binding
U	0042802	molecular_function	identical protein binding
U	0043101	biological_process	purine-containing compound salvage
U	0046059	biological_process	dAMP catabolic process
U	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
U	0047975	molecular_function	guanosine phosphorylase activity
U	0070062	cellular_component	extracellular exosome
U	1904813	cellular_component	ficolin-1-rich granule lumen
Y	0000255	biological_process	allantoin metabolic process
Y	0001882	molecular_function	nucleoside binding
Y	0002060	molecular_function	purine nucleobase binding
Y	0003824	molecular_function	catalytic activity
Y	0004731	molecular_function	purine-nucleoside phosphorylase activity
Y	0005515	molecular_function	protein binding
Y	0005576	cellular_component	extracellular region
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0006139	biological_process	nucleobase-containing compound metabolic process
Y	0006148	biological_process	inosine catabolic process
Y	0006149	biological_process	deoxyinosine catabolic process
Y	0006157	biological_process	deoxyadenosine catabolic process
Y	0006166	biological_process	purine ribonucleoside salvage
Y	0006204	biological_process	IMP catabolic process
Y	0006738	biological_process	nicotinamide riboside catabolic process
Y	0006955	biological_process	immune response
Y	0009116	biological_process	nucleoside metabolic process
Y	0009165	biological_process	nucleotide biosynthetic process
Y	0009410	biological_process	response to xenobiotic stimulus
Y	0016757	molecular_function	glycosyltransferase activity
Y	0016763	molecular_function	pentosyltransferase activity
Y	0032743	biological_process	positive regulation of interleukin-2 production
Y	0034418	biological_process	urate biosynthetic process
Y	0034774	cellular_component	secretory granule lumen
Y	0042102	biological_process	positive regulation of T cell proliferation
Y	0042301	molecular_function	phosphate ion binding
Y	0042802	molecular_function	identical protein binding
Y	0043101	biological_process	purine-containing compound salvage
Y	0046059	biological_process	dAMP catabolic process
Y	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
Y	0047975	molecular_function	guanosine phosphorylase activity
Y	0070062	cellular_component	extracellular exosome
Y	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE IM5 E 290

Chain	Residue
E	TYR88
E	THR242
E	ASN243
E	HIS257
E	VAL260
E	PO4291
E	ALA116
E	ALA117
E	GLY118
E	PHE200
E	GLU201
E	VAL217
E	GLY218
E	MET219

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 E 291

Chain	Residue
E	GLY32
E	SER33
E	HIS64
E	ARG84
E	HIS86
E	ASN115
E	ALA116
E	SER220
E	IM5290
E	HOH296

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 E 292

Chain	Residue
E	MET1
E	ARG148
Q	PRO92
Q	GLN144
Q	HOH306

site_id	AC4
Number of Residues	16
Details	BINDING SITE FOR RESIDUE IM5 Q 290

Chain	Residue
E	PHE159
Q	TYR88
Q	ALA116
Q	ALA117
Q	GLY118
Q	PHE200
Q	GLU201
Q	VAL217
Q	GLY218
Q	MET219
Q	THR242
Q	ASN243
Q	VAL245
Q	HIS257
Q	VAL260
Q	PO4291

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 Q 291

Chain	Residue
Q	GLY32
Q	SER33
Q	HIS64
Q	ARG84
Q	HIS86
Q	ASN115
Q	ALA116
Q	SER220
Q	IM5290
Q	HOH301

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 Q 292

Chain	Residue
Q	MET1
Q	ARG148
Y	PRO92
Y	GLN144

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 S 290

Chain	Residue
S	PRO92
S	GLN144
U	ARG148

site_id	AC8
Number of Residues	15
Details	BINDING SITE FOR RESIDUE IM5 S 291

Chain	Residue
S	SER33
S	TYR88
S	ALA116
S	GLY118
S	PHE200
S	GLU201
S	VAL217
S	GLY218
S	MET219
S	THR242
S	ASN243
S	HIS257
S	VAL260
S	PO4292
U	PHE159

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 S 292

Chain	Residue
S	GLY32
S	SER33
S	HIS64
S	ARG84
S	HIS86
S	ASN115
S	ALA116
S	SER220
S	IM5291
S	HOH299

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 T 290

Chain	Residue
T	MET1
T	ARG148
U	PRO92
U	GLN144

site_id	BC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE IM5 T 291

Chain	Residue
T	GLU201
T	VAL217
T	GLY218
T	MET219
T	THR242
T	ASN243
T	VAL245
T	HIS257
T	PO4292
S	PHE159
T	HIS86
T	TYR88
T	ALA116
T	ALA117
T	GLY118
T	VAL195
T	PHE200

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 T 292

Chain	Residue
T	GLY32
T	SER33
T	HIS64
T	ARG84
T	HIS86
T	ASN115
T	ALA116
T	SER220
T	IM5291

site_id	BC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE IM5 U 290

Chain	Residue
T	PHE159
U	TYR88
U	ALA116
U	ALA117
U	GLY118
U	PHE200
U	GLU201
U	VAL217
U	GLY218
U	MET219
U	THR242
U	ASN243
U	VAL245
U	HIS257
U	PO4291

site_id	BC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 U 291

Chain	Residue
U	GLY32
U	SER33
U	HIS64
U	ARG84
U	HIS86
U	ASN115
U	ALA116
U	SER220
U	IM5290
U	HOH297

site_id	BC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 Y 290

Chain	Residue
E	PRO92
E	GLN144
Y	MET1
Y	ARG148
Y	HOH303

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PO4 Y 291

Chain	Residue
E	ARG207
Y	ARG133

site_id	BC8
Number of Residues	18
Details	BINDING SITE FOR RESIDUE IM5 Y 292

Chain	Residue
Q	PHE159
Y	HIS86
Y	TYR88
Y	ALA116
Y	ALA117
Y	GLY118
Y	VAL195
Y	PHE200
Y	GLU201
Y	VAL217
Y	GLY218
Y	MET219
Y	THR242
Y	ASN243
Y	VAL245
Y	HIS257
Y	VAL260
Y	PO4293

site_id	BC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 Y 293

Chain	Residue
Y	GLY32
Y	SER33
Y	HIS64
Y	ARG84
Y	HIS86
Y	ASN115
Y	ALA116
Y	SER220
Y	IM5292
Y	HOH296

Functional Information from PROSITE/UniProt

site_id	PS01240
Number of Residues	42
Details	PNP_MTAP_2 Purine and other phosphorylases family 2 signature. VmmqGrfHmYegypLwkvTfpVrVfhllGvdt.LVvtNAaGGL

Chain	Residue	Details
E	VAL79-LEU120

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:23438750, ECO:0000305\|PubMed:14706628, ECO:0000305\|PubMed:1763067, ECO:0007744\|PDB:1RCT, ECO:0007744\|PDB:1ULA, ECO:0007744\|PDB:1ULB, ECO:0007744\|PDB:4EAR, ECO:0007744\|PDB:4EB8, ECO:0007744\|PDB:4GKA

Chain	Residue	Details
E	SER33
Q	SER33
S	SER33
T	SER33
U	SER33
Y	SER33

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P55859

Chain	Residue	Details
E	HIS64
Q	HIS64
S	HIS64
T	HIS64
U	HIS64
Y	HIS64

site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:23438750, ECO:0000305\|PubMed:14706628, ECO:0000305\|PubMed:1763067, ECO:0007744\|PDB:1RCT, ECO:0007744\|PDB:1ULA, ECO:0007744\|PDB:1ULB, ECO:0007744\|PDB:1V3Q, ECO:0007744\|PDB:4EAR, ECO:0007744\|PDB:4EB8, ECO:0007744\|PDB:4GKA

Chain	Residue	Details
E	ARG84
Q	ARG84
S	ARG84
T	ARG84
U	ARG84
Y	ARG84

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269\|PubMed:14706628, ECO:0000269\|PubMed:23438750, ECO:0007744\|PDB:1RCT, ECO:0007744\|PDB:4EAR, ECO:0007744\|PDB:4EB8

Chain	Residue	Details
E	TYR88
Q	TYR88
S	TYR88
T	TYR88
U	TYR88
Y	TYR88

site_id	SWS_FT_FI5
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269\|PubMed:14706628, ECO:0000269\|PubMed:23438750, ECO:0000305\|PubMed:1763067, ECO:0007744\|PDB:1RCT, ECO:0007744\|PDB:1ULB, ECO:0007744\|PDB:4EAR, ECO:0007744\|PDB:4EB8, ECO:0007744\|PDB:4GKA

Chain	Residue	Details
E	ALA116
S	GLU201
S	SER220
S	ASN243
T	ALA116
T	GLU201
T	SER220
T	ASN243
U	ALA116
U	GLU201
U	SER220
E	GLU201
U	ASN243
Y	ALA116
Y	GLU201
Y	SER220
Y	ASN243
E	SER220
E	ASN243
Q	ALA116
Q	GLU201
Q	SER220
Q	ASN243
S	ALA116

site_id	SWS_FT_FI6
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:14706628, ECO:0007744\|PDB:1RCT

Chain	Residue	Details
E	MET219
U	HIS257
Y	MET219
Y	HIS257
E	HIS257
Q	MET219
Q	HIS257
S	MET219
S	HIS257
T	MET219
T	HIS257
U	MET219

site_id	SWS_FT_FI7
Number of Residues	6
Details	SITE: Important for substrate specificity => ECO:0000269\|PubMed:9305964

Chain	Residue	Details
E	ASN243
Q	ASN243
S	ASN243
T	ASN243
U	ASN243
Y	ASN243

site_id	SWS_FT_FI8
Number of Residues	6
Details	MOD_RES: N-acetylmethionine => ECO:0000269\|Ref.8, ECO:0007744\|PubMed:19413330, ECO:0007744\|PubMed:22223895

Chain	Residue	Details
E	MET1
Q	MET1
S	MET1
T	MET1
U	MET1
Y	MET1

site_id	SWS_FT_FI9
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
E	SER251
Q	SER251
S	SER251
T	SER251
U	SER251
Y	SER251

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
E	SER33	hydrogen bond donor
E	HIS257	electrostatic stabiliser, hydrogen bond acceptor
E	HIS64	electrostatic stabiliser
E	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	TYR88	electrostatic stabiliser, hydrogen bond donor
E	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
E	ALA116	electrostatic stabiliser, hydrogen bond donor
E	MET219	electrostatic stabiliser, hydrogen bond donor
E	SER220	electrostatic stabiliser, hydrogen bond donor
E	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

site_id	MCSA2
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
Q	SER33	hydrogen bond donor
Q	HIS257	electrostatic stabiliser, hydrogen bond acceptor
Q	HIS64	electrostatic stabiliser
Q	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
Q	TYR88	electrostatic stabiliser, hydrogen bond donor
Q	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
Q	ALA116	electrostatic stabiliser, hydrogen bond donor
Q	MET219	electrostatic stabiliser, hydrogen bond donor
Q	SER220	electrostatic stabiliser, hydrogen bond donor
Q	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

site_id	MCSA3
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
S	SER33	hydrogen bond donor
S	HIS257	electrostatic stabiliser, hydrogen bond acceptor
S	HIS64	electrostatic stabiliser
S	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
S	TYR88	electrostatic stabiliser, hydrogen bond donor
S	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
S	ALA116	electrostatic stabiliser, hydrogen bond donor
S	MET219	electrostatic stabiliser, hydrogen bond donor
S	SER220	electrostatic stabiliser, hydrogen bond donor
S	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

site_id	MCSA4
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
T	SER33	hydrogen bond donor
T	HIS257	electrostatic stabiliser, hydrogen bond acceptor
T	HIS64	electrostatic stabiliser
T	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
T	TYR88	electrostatic stabiliser, hydrogen bond donor
T	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
T	ALA116	electrostatic stabiliser, hydrogen bond donor
T	MET219	electrostatic stabiliser, hydrogen bond donor
T	SER220	electrostatic stabiliser, hydrogen bond donor
T	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

site_id	MCSA5
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
U	SER33	hydrogen bond donor
U	HIS257	electrostatic stabiliser, hydrogen bond acceptor
U	HIS64	electrostatic stabiliser
U	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
U	TYR88	electrostatic stabiliser, hydrogen bond donor
U	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
U	ALA116	electrostatic stabiliser, hydrogen bond donor
U	MET219	electrostatic stabiliser, hydrogen bond donor
U	SER220	electrostatic stabiliser, hydrogen bond donor
U	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

site_id	MCSA6
Number of Residues	10
Details	M-CSA 17

Chain	Residue	Details
Y	SER33	hydrogen bond donor
Y	HIS257	electrostatic stabiliser, hydrogen bond acceptor
Y	HIS64	electrostatic stabiliser
Y	HIS86	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
Y	TYR88	electrostatic stabiliser, hydrogen bond donor
Y	GLU89	activator, electrostatic stabiliser, hydrogen bond acceptor
Y	ALA116	electrostatic stabiliser, hydrogen bond donor
Y	MET219	electrostatic stabiliser, hydrogen bond donor
Y	SER220	electrostatic stabiliser, hydrogen bond donor
Y	ASN243	electrostatic stabiliser, hydrogen bond donor, polar interaction

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)