8ENT

Interleukin-21 signaling complex with IL-21R and IL-2Rg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	66.167, 66.464, 162.684
Unit cell angles	83.34, 83.71, 73.10

Refinement procedure

Resolution	48.170 - 2.830
R-factor	0.2551
Rwork	0.254
R-free	0.29970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgx 2b5i
RMSD bond length	0.002
RMSD bond angle	0.589
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	2.932
High resolution limit [Å]	2.830	2.831
Rpim	0.129
Number of reflections	57754	5757
<I/σ(I)>	4.22
Completeness [%]	91.7
Redundancy	3.7
CC(1/2)	0.985	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.1M Tris pH 8, 20% PEG 6000