8ENT

Interleukin-21 signaling complex with IL-21R and IL-2Rg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	Interleukin-21	polymer	144	16704.0	4	UniProt (Q9HBE4) Pfam (PF02372) In PDB	Homo sapiens (human)	IL-21,Za11
2	B, E, H, K	Interleukin-21 receptor	polymer	237	27465.5	4	UniProt (Q9HBE5) In PDB	Homo sapiens (human)	IL-21 receptor,IL-21R,Novel interleukin receptor
3	C, F, I	Cytokine receptor common subunit gamma	polymer	207	24951.8	3	UniProt (P31785) Pfam (PF21604) Pfam (PF21605) In PDB	Homo sapiens (human)	Interleukin-2 receptor subunit gamma,IL-2 receptor subunit gamma,IL-2R subunit gamma,IL-2RG,gammaC,p64
4	L, M, O	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	N	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	I, C, E, F, H	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
7	I, K, C, D, E...	1,2-ETHANEDIOL	non-polymer		62.1	14	Chemie (EDO)
8		water	water		18.0	55	Chemie (HOH)

Sequence modifications

A, D, G, J: 1 - 133 (UniProt: Q9HBE4)

PDB	External Database	Details
Gln 68	Asn 97	engineered mutation
Gly 134	-	expression tag
Ala 135	-	expression tag
Pro 136	-	expression tag
Gly 137	-	expression tag
Ser 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag

B, E, H, K: 1 - 210 (UniProt: Q9HBE5)

PDB	External Database	Details
Gln 78	Asn 97	engineered mutation
Gln 85	Asn 104	engineered mutation
Asp 106	Asn 125	engineered mutation
Gln 116	Asn 135	engineered mutation
Lys 211	-	expression tag
Ala 212	-	expression tag
Ala 213	-	expression tag
Ser 214	-	expression tag
Gly 215	-	expression tag
Arg 216	-	expression tag
Gly 217	-	expression tag
Leu 218	-	expression tag
Asn 219	-	expression tag
Asp 220	-	expression tag
Ile 221	-	expression tag
Phe 222	-	expression tag
Glu 223	-	expression tag
Ala 224	-	expression tag
Gln 225	-	expression tag
Lys 226	-	expression tag
Ile 227	-	expression tag
Glu 228	-	expression tag
Trp 229	-	expression tag
His 230	-	expression tag
Glu 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag

C, F, I: 33 - 232 (UniProt: P31785)

PDB	External Database	Details
Pro 32	-	expression tag
Gln 53	Asn 75	engineered mutation
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	251533.4
Branched	Number of molecules	4
	Total formula weight	1859.7
Non-Polymers*	Number of molecules	21
	Total formula weight	2417.4
All*	Total formula weight	255810.5

*Water molecules are not included.