8EBN

Structure of KLHDC2-EloB/C tetrameric assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-30
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 1 2
Unit cell lengths	142.431, 142.431, 140.479
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.620 - 2.600
R-factor	0.2131
Rwork	0.211
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vcb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.067	0.030	0.613
Rmeas	0.077	0.034	0.705
Rpim	0.037	0.016	0.345
Total number of observations	197411
Number of reflections	49565	5043	4948
<I/σ(I)>	11.5
Completeness [%]	98.6	97.5	99.6
Redundancy	4	4	4
CC(1/2)		0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	% PEG3350, 0.2 M KSCN, 0.1 M Tris, 0.1M Na/K Tartrate, 2.5% MPD, pH =6.8