8DYP
Crystal structure of human cystine transporter cystinosin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.510, 71.270, 194.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.190 - 3.400 |
R-factor | 0.2721 |
Rwork | 0.270 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p0g 4x5n |
RMSD bond length | 0.003 |
RMSD bond angle | 0.724 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.190 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 9815 | 886 |
<I/σ(I)> | 15.2 | 1.69 |
Completeness [%] | 97.3 | 88.9 |
Redundancy | 14.9 | 8.3 |
CC(1/2) | 0.967 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293.15 | 12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0 |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293.15 | 12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0 |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293.15 | 12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0 |