8ASC

Ku70/80 binds to the Ku-binding motif of PAXX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	85.024, 428.570, 96.065
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	45.320 - 2.950
R-factor	0.2547
Rwork	0.252
R-free	0.29810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jey
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.734	3.588
High resolution limit [Å]	2.950	2.973
Rmerge	0.255	1.661
Rmeas	0.273	1.806
Rpim	0.098	0.695
Number of reflections	34200	1710
<I/σ(I)>	6	1.5
Completeness [%]	86.7	53.4
Redundancy	7.8	6.6
CC(1/2)	0.992	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	12% PEG- 3350; 0.1 M Bis-Tris Propane pH 8.5; 0.3 M Ammonium Sulfate