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8ASC

Ku70/80 binds to the Ku-binding motif of PAXX

Summary for 8ASC
Entry DOI10.2210/pdb8asc/pdb
DescriptorX-ray repair cross-complementing protein 6, X-ray repair cross-complementing protein 5, DNA (5'-D(P*CP*GP*GP*AP*TP*CP*GP*AP*GP*GP*GP*CP*CP*CP*GP*AP*TP*AP*T)-3'), ... (7 entities in total)
Functional Keywordsnhej, dna repair, dna binding protein
Biological sourceHomo sapiens (human)
More
Total number of polymer chains18
Total formula weight573910.75
Authors
Seif El Dahan, M.,Ropars, V.,Charbonnier, J.B. (deposition date: 2022-08-19, release date: 2023-06-21, Last modification date: 2024-10-16)
Primary citationSeif-El-Dahan, M.,Kefala-Stavridi, A.,Frit, P.,Hardwick, S.W.,Chirgadze, D.Y.,Maia De Oliviera, T.,Britton, S.,Barboule, N.,Bossaert, M.,Pandurangan, A.P.,Meek, K.,Blundell, T.L.,Ropars, V.,Calsou, P.,Charbonnier, J.B.,Chaplin, A.K.
PAXX binding to the NHEJ machinery explains functional redundancy with XLF.
Sci Adv, 9:eadg2834-eadg2834, 2023
Cited by
PubMed Abstract: Nonhomologous end joining is a critical mechanism that repairs DNA double-strand breaks in human cells. In this work, we address the structural and functional role of the accessory protein PAXX [paralog of x-ray repair cross-complementing protein 4 (XRCC4) and XRCC4-like factor (XLF)] in this mechanism. Here, we report high-resolution cryo-electron microscopy (cryo-EM) and x-ray crystallography structures of the PAXX C-terminal Ku-binding motif bound to Ku70/80 and cryo-EM structures of PAXX bound to two alternate DNA-dependent protein kinase (DNA-PK) end-bridging dimers, mediated by either Ku80 or XLF. We identify residues critical for the Ku70/PAXX interaction in vitro and in cells. We demonstrate that PAXX and XLF can bind simultaneously to the Ku heterodimer and act as structural bridges in alternate forms of DNA-PK dimers. Last, we show that engagement of both proteins provides a complementary advantage for DNA end synapsis and end joining in cells.
PubMed: 37256950
DOI: 10.1126/sciadv.adg2834
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.95 Å)
Structure validation

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