8DYP

Crystal structure of human cystine transporter cystinosin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 2 21 21
Unit cell lengths	49.510, 71.270, 194.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.190 - 3.400
R-factor	0.2721
Rwork	0.270
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p0g 4x5n
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.190	3.520
High resolution limit [Å]	3.400	3.400
Number of reflections	9815	886
<I/σ(I)>	15.2	1.69
Completeness [%]	97.3	88.9
Redundancy	14.9	8.3
CC(1/2)	0.967	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293.15	12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0
1	LIPIDIC CUBIC PHASE	6.5	293.15	12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0
1	LIPIDIC CUBIC PHASE	6.5	293.15	12% PEG400, 80mM Li2SO4, and 50mM MES pH6.0