7ZXK
Human IL-27 in complex with neutralizing SRF388 FAb fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-25 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.806, 104.622, 121.754 |
Unit cell angles | 90.00, 98.65, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 120.400 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.060 | 2.081 |
Number of reflections | 108947 | 8013 |
<I/σ(I)> | 14.82 | 0.92 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5.23 | 5.34 |
CC(1/2) | 0.999 | 0.711 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2 M ammonium citrate dibasic, 2.5 % glycerol |