7Z1X
Crystal structure of human Gasdermin D complexed with nanobodies VHH-2 and VHH-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976255 |
Spacegroup name | P 31 |
Unit cell lengths | 108.353, 108.353, 124.041 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.840 | 1.926 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.016 | 0.855 |
Rmeas | 0.023 | |
Rpim | 0.016 | 0.855 |
Number of reflections | 136585 | 13351 |
<I/σ(I)> | 17.43 | 0.73 |
Completeness [%] | 99.7 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.443 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 293 | 20 mg/ml protein complex; 0.04 M NaCl, 25.8% (v/v) PEG400, 0.05 M Na3Cit pH 4.4 |