7YZE

Crystal structure of the human FoxA2 bound to the TGTTTACT site (forkhead motif GTAAACA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	C 2 2 21
Unit cell lengths	46.035, 92.369, 118.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 1.990
R-factor	0.2087
Rwork	0.208
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x07
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.185	2.126
High resolution limit [Å]	1.985	1.985
Rpim	0.012	0.584
Number of reflections	15071	756
<I/σ(I)>	26.3
Completeness [%]	93.9	53.5
Redundancy	13.1
CC(1/2)	0.999	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5