7YZE
Crystal structure of the human FoxA2 bound to the TGTTTACT site (forkhead motif GTAAACA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.035, 92.369, 118.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.180 - 1.990 |
R-factor | 0.2087 |
Rwork | 0.208 |
R-free | 0.21510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x07 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.185 | 2.126 |
High resolution limit [Å] | 1.985 | 1.985 |
Rpim | 0.012 | 0.584 |
Number of reflections | 15071 | 756 |
<I/σ(I)> | 26.3 | |
Completeness [%] | 93.9 | 53.5 |
Redundancy | 13.1 | |
CC(1/2) | 0.999 | 0.573 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5 |