7WL0
Crystal structure of human ALKBH5 in complex with N-oxalylglycine (NOG) and m6A-containing ssRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.770, 132.370, 77.960 |
Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
Resolution | 58.670 - 2.500 |
R-factor | 0.1985 |
Rwork | 0.196 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v4g |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.250 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.489 | 2.437 |
Number of reflections | 41144 | 4103 |
<I/σ(I)> | 4.76 | 1.17 |
Completeness [%] | 99.9 | 99.81 |
Redundancy | 14 | 11.8 |
CC(1/2) | 0.981 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium sulfate decahydrate, 20% (w/v) PEG 3350 |