7Q9U
Crystal structure of the high affinity KRas mutant PDE6D complex
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97628 |
Spacegroup name | P 1 |
Unit cell lengths | 51.086, 57.925, 78.913 |
Unit cell angles | 82.77, 81.71, 68.45 |
Refinement procedure
Resolution | 53.706 - 2.238 |
Rwork | 0.199 |
R-free | 0.25450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tb5 |
RMSD bond length | 0.001 |
RMSD bond angle | 1.609 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.706 | 2.300 |
High resolution limit [Å] | 2.238 | 2.240 |
Number of reflections | 37781 | 2327 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 94.7 | |
Redundancy | 1.7 | |
CC(1/2) | 0.995 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium sulphate, 0.1 M tri sodium citrate pH 5.6, 15 % w/v PEG 4000 |