7PL1
Crystal structure of human METTL1 bound to Sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-12 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.760, 128.760, 39.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.720 - 1.850 |
R-factor | 0.1907 |
Rwork | 0.190 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ogj |
RMSD bond length | 0.008 |
RMSD bond angle | 0.975 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.19.2-4158-000) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.720 | 1.960 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 28966 | 4569 |
<I/σ(I)> | 29.82 | |
Completeness [%] | 99.4 | |
Redundancy | 15.8 | |
CC(1/2) | 1.000 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 200 mM lithium sulfate, 20% PEG 1000, 100 mM phosphate-citrate buffer pH 4.2 |