7NOZ
Structure of the nanobody stablized properdin bound alternative pathway proconvertase C3b:FB:FP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 148.690, 179.460, 192.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.590 - 3.900 |
| R-factor | 0.2494 |
| Rwork | 0.249 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xwb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.590 | 4.039 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.195 | |
| Number of reflections | 47647 | 4688 |
| <I/σ(I)> | 10.84 | 0.84 |
| Completeness [%] | 99.8 | 99.91 |
| Redundancy | 39.7 | 43.8 |
| CC(1/2) | 0.998 | 0.392 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Na-acetate pH 5.3, 0.1 M Mg-formate,7% PEG5000 MME |
