7MYZ

Structure of the full length 5-TM receptor CD47 bound to Fab B6H12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	141.535, 53.734, 181.697
Unit cell angles	90.00, 92.88, 90.00

Refinement procedure

Resolution	48.680 - 3.400
R-factor	0.2539
Rwork	0.253
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzu
RMSD bond length	0.010
RMSD bond angle	1.628
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.480
High resolution limit [Å]	3.400	8.370	3.400
Rmerge	0.358	0.186	0.959
Rmeas	0.417	0.216	1.155
Rpim	0.206	0.106	0.624
Total number of observations	118336
Number of reflections	35403	2606	1986
<I/σ(I)>	3.7
Completeness [%]	92.1	95.4	79.3
Redundancy	3.3	3.6	2.5
CC(1/2)		0.940	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	100mM sodium citrate pH 6.0, 450mM ammonium acetate, 32% PEG400