7LWA
Human Exonuclease 5 crystal structure (T88E) in complex with ssDNA and Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 50.512, 84.722, 95.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.517 - 2.850 |
R-factor | 0.2058 |
Rwork | 0.203 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lw7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.431 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.520 | 47.520 | 3.000 |
High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
Rmerge | 0.362 | 0.062 | 2.352 |
Rmeas | 0.377 | 0.065 | 2.443 |
Rpim | 0.103 | 0.019 | 0.655 |
Number of reflections | 9998 | 376 | 1423 |
<I/σ(I)> | 8.1 | 27.6 | 1.4 |
Completeness [%] | 99.7 | 99.7 | 99.6 |
Redundancy | 13 | 10.9 | 13.5 |
CC(1/2) | 0.992 | 0.999 | 0.605 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350 and 0.1 M magnesium formate |