7LF4
Structure of importin a3 bound to the p50- and p65-NLSs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.130, 117.130, 210.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.980 - 2.850 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tbk |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rpim | 0.082 | 0.712 |
| Number of reflections | 34874 | 1835 |
| <I/σ(I)> | 6.3 | 1.2 |
| Completeness [%] | 85.4 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.994 | 0.287 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 25% PEG 3350 |
