7KMA
Crystal structure of eif2Balpha with a ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.200, 155.540, 140.110 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 55.610 - 2.700 |
| R-factor | 0.1933 |
| Rwork | 0.191 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ecs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_3916) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.610 | 2.797 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.036 | 0.320 |
| Number of reflections | 150142 | 15063 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 93.7 | 99.64 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 12% PEG 4000, 100 mM sodium acetate, 100 mM ammonium sulfate, 0.5% octyl-beta-glucoside, pH 4.6 |
