7JUK

Crystal structure of PTEN with a tetra-phosphorylated tail (4p-crPTEN-13sp-T2, SDTTDSDPENEG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	277
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	I 4
Unit cell lengths	113.444, 113.444, 57.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.520 - 3.150
R-factor	0.1887
Rwork	0.184
R-free	0.28890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5r
RMSD bond length	0.005
RMSD bond angle	1.408
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.217	28.503	3.320
High resolution limit [Å]	3.150	9.960	3.150
Rmerge		0.086	0.715
Rmeas	0.152	0.093	0.779
Rpim	0.059	0.035	0.306
Total number of observations	41598	1424	5502
Number of reflections	6346	213	878
<I/σ(I)>	8.8	19.7	2.7
Completeness [%]	99.3	96.6	95.9
Redundancy	6.6	6.7	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.2 M DL-malic acid