7DC8
Crystal structure of Switch Ab Fab and hIL6R in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-12-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 103.043, 185.612, 152.085 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.091 - 2.757 |
R-factor | 0.2107 |
Rwork | 0.207 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n26 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14rc2_3184) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.810 | 92.810 | 2.900 |
High resolution limit [Å] | 2.750 | 8.690 | 2.750 |
Rmerge | 0.107 | 0.030 | 0.818 |
Rmeas | 0.111 | 0.032 | 0.851 |
Rpim | 0.030 | 0.009 | 0.232 |
Total number of observations | 510514 | ||
Number of reflections | 38295 | 1328 | 5522 |
<I/σ(I)> | 20 | ||
Completeness [%] | 100.0 | 99.9 | 99.7 |
Redundancy | 13.3 | 12 | 13.3 |
CC(1/2) | 0.999 | 1.000 | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 294 | 0.06 M Tris, 12.0 %(w/v) Polyethylene glycol 1500, 0.06 M Ammonium sulfate |