7BRE

The crystal structure of MLL2 in complex with ASH2L and RBBP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	79.075, 66.195, 83.734
Unit cell angles	90.00, 93.23, 90.00

Refinement procedure

Resolution	37.833 - 2.803
R-factor	0.2209
Rwork	0.218
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3toj 5f6e
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.159	0.081	0.642
Rmeas	0.173	0.088	0.700
Rpim	0.066	0.034	0.276
Total number of observations	142941
Number of reflections	21457	2216	2120
<I/σ(I)>	3.4
Completeness [%]	100.0	99.9	100
Redundancy	6.7	6.4	6.3
CC(1/2)		0.997	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M Ammonium tartrate dibasic pH 7.0, 12%(w/v) Polyethylene glycol 3,350