7BO7

CRYSTAL STRUCTURE OF THE HUMAN PRMT5:MEP50 COMPLEX with JNJB44355437

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91741
Spacegroup name	I 2 2 2
Unit cell lengths	101.190, 137.250, 179.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.367 - 2.830
Rwork	0.205
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rlq
RMSD bond length	0.007
RMSD bond angle	1.568
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.367	2.980
High resolution limit [Å]	2.830	2.830
Rmerge		1.379
Number of reflections	30068	4286
<I/σ(I)>	10	0.9
Completeness [%]	99.7	99.1
Redundancy	6.2
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG3350, 150mM ammonium sulfate