7BO7

CRYSTAL STRUCTURE OF THE HUMAN PRMT5:MEP50 COMPLEX with JNJB44355437

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AAA)	Protein arginine N-methyltransferase 5	polymer	645	73763.6	1	UniProt (O14744) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286)	Homo sapiens (Human)	72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog,SKB1Hs
2	B (BBB)	Methylosome protein 50	polymer	348	37586.1	1	UniProt (Q9BQA1) Pfam (PF00400)	Homo sapiens (Human)	MEP-50,Androgen receptor cofactor p44,WD repeat-containing protein 77,p44/Mep50
3	C (AAA)	(2~{R},3~{R},4~{S},5~{R})-2-[(4~{a}~{S},7~{a}~{S})-4-azanyl-1,4,4~{a},7~{a}-tetrahydropyrrolo[2,3-d]pyrimidin-7-yl]-5-(quinolin-7-yloxymethyl)oxolane-3,4-diol	non-polymer		397.4	1	Chemie (U6K)
4	D (AAA)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
5	E, F, G (AAA)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
6	H (AAA)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	I, J (AAA, BBB)	water	water		18.0	52	Chemie (HOH)

Sequence modifications

AAA: 2 - 637 (UniProt: O14744)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

BBB: 2 - 342 (UniProt: Q9BQA1)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111349.7
Non-Polymers*	Number of molecules	6
	Total formula weight	928.1
All*	Total formula weight	112277.8

*Water molecules are not included.