7AHZ
Crystal structure of Western clawed frog MDM2 RING domain homodimer bound to UbcH5B-Ub
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.030, 153.150, 82.080 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 52.623 - 1.820 |
| Rwork | 0.195 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mnj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.462 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.623 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 113701 | 5647 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1 M HEPES, 10 % w/v PEG 20000 |
