7AHZ

Crystal structure of Western clawed frog MDM2 RING domain homodimer bound to UbcH5B-Ub

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, DDD, GGG, JJJ	E3 ubiquitin-protein ligase Mdm2	polymer	71	7908.4	4	UniProt (Q6P3Q9) Pfam (PF13920) In PDB	Xenopus tropicalis (Western clawed frog)
2	BBB, EEE, HHH, KKK	Ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)	polymer	147	16851.4	4	UniProt (Q28EY6) Pfam (PF00179) In PDB	Xenopus tropicalis (Western clawed frog)	Ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog)
3	CCC, FFF, III, LLL	Polyubiquitin-B	polymer	81	8922.1	4	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
4	AAA, DDD, GGG, JJJ	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
5	HHH	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	356	Chemie (HOH)

Sequence modifications

AAA, DDD, GGG, JJJ: 414 - 482 (UniProt: Q6P3Q9)

PDB	External Database	Details
Gly 412	-	expression tag
Ser 413	-	expression tag

BBB, EEE, HHH, KKK: 1 - 147 (UniProt: Q28EY6)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

CCC, FFF, III, LLL: -2 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Ser 0	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	134727.8
Non-Polymers*	Number of molecules	9
	Total formula weight	558.7
All*	Total formula weight	135286.5

*Water molecules are not included.