7ZXK

Human IL-27 in complex with neutralizing SRF388 FAb fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	86.806, 104.622, 121.754
Unit cell angles	90.00, 98.65, 90.00

Refinement procedure

Resolution	60.180 - 2.200
R-factor	0.1862
Rwork	0.184
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6azm 5ijk 5fuc
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.400	2.260
High resolution limit [Å]	2.200	2.200
Rmeas	0.060	2.081
Number of reflections	108947	8013
<I/σ(I)>	14.82	0.92
Completeness [%]	99.8	99.6
Redundancy	5.23	5.34
CC(1/2)	0.999	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M ammonium citrate dibasic, 2.5 % glycerol