7WN8

Crystal structure of antibody (BC31M5) binds to CD47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	91.402, 94.281, 174.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 2.800
R-factor	0.2451
Rwork	0.243
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzt 4jpk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASES
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.590	2.901
High resolution limit [Å]	2.800	2.801
Rmerge	0.161
Rmeas	0.168
Rpim	0.046
Number of reflections	37875	3664
<I/σ(I)>	16.96
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.997	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291.15	0.2M Zinc acetate dihydrate, 0.1M Sodium cacodylate trihydrate (pH 6.5), 18% w/v PEG 8000