7WL0

Crystal structure of human ALKBH5 in complex with N-oxalylglycine (NOG) and m6A-containing ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	59.770, 132.370, 77.960
Unit cell angles	90.00, 100.99, 90.00

Refinement procedure

Resolution	58.670 - 2.500
R-factor	0.1985
Rwork	0.196
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v4g
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.250	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.489	2.437
Number of reflections	41144	4103
<I/σ(I)>	4.76	1.17
Completeness [%]	99.9	99.81
Redundancy	14	11.8
CC(1/2)	0.981	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium sulfate decahydrate, 20% (w/v) PEG 3350