7Q9U

Crystal structure of the high affinity KRas mutant PDE6D complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97628
Spacegroup name	P 1
Unit cell lengths	51.086, 57.925, 78.913
Unit cell angles	82.77, 81.71, 68.45

Refinement procedure

Resolution	53.706 - 2.238
Rwork	0.199
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tb5
RMSD bond length	0.001
RMSD bond angle	1.609
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.706	2.300
High resolution limit [Å]	2.238	2.240
Number of reflections	37781	2327
<I/σ(I)>	6.1
Completeness [%]	94.7
Redundancy	1.7
CC(1/2)	0.995	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium sulphate, 0.1 M tri sodium citrate pH 5.6, 15 % w/v PEG 4000