7PL1
Crystal structure of human METTL1 bound to Sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.760, 128.760, 39.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.720 - 1.850 |
| R-factor | 0.1907 |
| Rwork | 0.190 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ogj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.975 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.19.2-4158-000) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 28966 | 4569 |
| <I/σ(I)> | 29.82 | |
| Completeness [%] | 99.4 | |
| Redundancy | 15.8 | |
| CC(1/2) | 1.000 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 200 mM lithium sulfate, 20% PEG 1000, 100 mM phosphate-citrate buffer pH 4.2 |
