7MYZ
Structure of the full length 5-TM receptor CD47 bound to Fab B6H12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 141.535, 53.734, 181.697 |
| Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
| Resolution | 48.680 - 3.400 |
| R-factor | 0.2539 |
| Rwork | 0.253 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tzu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.480 |
| High resolution limit [Å] | 3.400 | 8.370 | 3.400 |
| Rmerge | 0.358 | 0.186 | 0.959 |
| Rmeas | 0.417 | 0.216 | 1.155 |
| Rpim | 0.206 | 0.106 | 0.624 |
| Total number of observations | 118336 | ||
| Number of reflections | 35403 | 2606 | 1986 |
| <I/σ(I)> | 3.7 | ||
| Completeness [%] | 92.1 | 95.4 | 79.3 |
| Redundancy | 3.3 | 3.6 | 2.5 |
| CC(1/2) | 0.940 | 0.456 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 293 | 100mM sodium citrate pH 6.0, 450mM ammonium acetate, 32% PEG400 |
